Elevated tumor necrosis factor-alpha expression in periapical lesions infected by epstein-barr virus

Katinka Hernádi, Eszter Gyöngyösi, Beáta Mészáros, Levente Szakács, Anita Szalmás, Eszter Csoma, Richárd Mogyorósi, Levente Czompa, György Veress, István Varga, Ildikó Judit Márton, József Kónya

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17 Citations (Scopus)

Abstract

Introduction: In apical periodontitis, there is an intense inflammatory response to endodontopathogenic bacteria, an essential component of the pathogenic microbiota. The inflammation can be aggravated by herpesviruses acting as nonessential pathogens in periapical lesions. This study aimed to determine the levels of tumor necrosis factor-alpha (TNF-α) and transforming growth factor-beta (TGF-β) in periapical lesions in relation to local occurrence of Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), human herpesvirus 6 (HHV-6), and human herpesvirus 8 (HHV-8). Methods: Fifty-eight samples with apical periodontitis and 20 clinically healthy gingival control tissues were collected. Viral DNA was determined with nested polymerase chain reaction, and cytokine mRNA expression was detected with real-time polymerase chain reaction assays. Results: Periapical lesions harbored EBV (75.9%) and HHV-6 (22.4%) at significantly higher frequencies compared with controls (P <.000001 and P <.05, respectively), whereas HCMV (12%) and HHV-8 (0%) occurred rarely. The median TNF-α expression was 13 times higher (P <.001) and TGF-β expression was 5 times higher in periapical lesions than in controls (P <.001). TNF-α expression was significantly higher in EBV-positive lesions than in EBV-negative lesions (P =.032). Presence of symptoms, lesion size, and infection by HCMV or HHV-6 had no significant association with either TNF-α or TGF-β expression. Conclusions: The herpesviral component of the endodontic microbiota did not correlate with TGF-β expression, whereas EBV infection was associated with a median 1.5 times further elevation of the high TNF-α expression characteristic for periapical lesions.

Original languageEnglish
Pages (from-to)456-460
Number of pages5
JournalJournal of Endodontics
Volume39
Issue number4
DOIs
Publication statusPublished - Apr 1 2013

Keywords

  • Epstein-Barr virus
  • TGF-beta
  • TNF-alpha
  • human cytomegalovirus
  • human herpesvirus 6
  • periapical lesion
  • real time PCR

ASJC Scopus subject areas

  • Dentistry(all)

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  • Cite this

    Hernádi, K., Gyöngyösi, E., Mészáros, B., Szakács, L., Szalmás, A., Csoma, E., Mogyorósi, R., Czompa, L., Veress, G., Varga, I., Márton, I. J., & Kónya, J. (2013). Elevated tumor necrosis factor-alpha expression in periapical lesions infected by epstein-barr virus. Journal of Endodontics, 39(4), 456-460. https://doi.org/10.1016/j.joen.2012.12.028