Elevated rate of Thelper1 (TH1) lymphocytes and serum IFN-γ levels in psoriatic patients

A. Szegedi, M. Aleksza, Andrea Gonda, Beatrix Irinyi, S. Sipka, J. Hunyadi, P. Antal-Szalmás

Research output: Contribution to journalArticle

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Abstract

Several disorders are known to be associated with altered Thelper1/Thelper2 (TH1/TH2) cytokine balance. Psoriasis is characterized by increased systemic and local production of TH1 and pro-inflammatory cytokines. Furthermore recent data indicate the dominant presence of TH1 lymphocytes in the circulation and TH1 and Tcytotoxic1 (TC1) cells in lesional skin of psoriatic patients. In order to assess the systemic TH1/TH2 imbalance in psoriasis most of the studies so far tested isolated peripheral mononuclear cells. As a new approach we applied a whole blood flow cytometric assay to determine the rate of circulating TH1/TH2 and TC1/Tcytotoxic2 (TC2) lymphocytes based on their intracellular IFN-γ, IL-4 and IL-10 expression. Besides, serum levels of these cytokines were determined in healthy controls and psoriatic patients by commercial ELISAs. In psoriatic patients we found significantly (P+/IFN-γ+ lymphocytes (30.3±8.8%) while the percent of CD4+/IL-4+ cells (0.37±0.31%) were significantly (P+/IFN-γ+: 20.1±7.3% and CD4+/IL-4+: 0.78±0.44%). The IL-10-positive CD4+ and CD8+ cells also had higher rate in psoriasis, but the difference between patients and controls was not significant, similarly to the rate of CD8+/IFN-γ+ and CD8+/IL-4+ lymphocytes. Beside cellular expression, serum IFN-γ levels were also significantly higher (control: 4.9±6.4 pg/ml; psoriatic patients: 35.9±47.0 pg/ml; PH1/TH2 balance in psoriasis measured in non-separated whole blood T cells.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalImmunology Letters
Volume86
Issue number3
DOIs
Publication statusPublished - May 1 2003

Fingerprint

Psoriasis
Interleukin-4
Lymphocytes
Serum
Cytokines
Interleukin-10
Blood Cells
Enzyme-Linked Immunosorbent Assay
T-Lymphocytes
Skin

Keywords

  • Cytotoxic T cells
  • Flow cytometry
  • Helper T cells
  • Intracellular cytokines
  • Whole blood

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Elevated rate of Thelper1 (TH1) lymphocytes and serum IFN-γ levels in psoriatic patients. / Szegedi, A.; Aleksza, M.; Gonda, Andrea; Irinyi, Beatrix; Sipka, S.; Hunyadi, J.; Antal-Szalmás, P.

In: Immunology Letters, Vol. 86, No. 3, 01.05.2003, p. 277-280.

Research output: Contribution to journalArticle

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abstract = "Several disorders are known to be associated with altered Thelper1/Thelper2 (TH1/TH2) cytokine balance. Psoriasis is characterized by increased systemic and local production of TH1 and pro-inflammatory cytokines. Furthermore recent data indicate the dominant presence of TH1 lymphocytes in the circulation and TH1 and Tcytotoxic1 (TC1) cells in lesional skin of psoriatic patients. In order to assess the systemic TH1/TH2 imbalance in psoriasis most of the studies so far tested isolated peripheral mononuclear cells. As a new approach we applied a whole blood flow cytometric assay to determine the rate of circulating TH1/TH2 and TC1/Tcytotoxic2 (TC2) lymphocytes based on their intracellular IFN-γ, IL-4 and IL-10 expression. Besides, serum levels of these cytokines were determined in healthy controls and psoriatic patients by commercial ELISAs. In psoriatic patients we found significantly (P+/IFN-γ+ lymphocytes (30.3±8.8{\%}) while the percent of CD4+/IL-4+ cells (0.37±0.31{\%}) were significantly (P+/IFN-γ+: 20.1±7.3{\%} and CD4+/IL-4+: 0.78±0.44{\%}). The IL-10-positive CD4+ and CD8+ cells also had higher rate in psoriasis, but the difference between patients and controls was not significant, similarly to the rate of CD8+/IFN-γ+ and CD8+/IL-4+ lymphocytes. Beside cellular expression, serum IFN-γ levels were also significantly higher (control: 4.9±6.4 pg/ml; psoriatic patients: 35.9±47.0 pg/ml; PH1/TH2 balance in psoriasis measured in non-separated whole blood T cells.",
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