Elevated osteopontin expression and proliferative/apoptotic ratio in the colorectal adenoma-dysplasia-carcinoma sequence

Gábor Valcz, F. Sípos, T. Krenács, Jeannette Molnár, Árpád V. Patai, Katalin Leiszter, Kinga Tóth, N. Solymosi, O. Galamb, B. Molnár, Z. Tulassay

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Colorectal cancer progression is characterized by altered epithelial proliferation and apoptosis and by changed expression of tumor development regulators. Our aims were to determine the proliferative/apoptotic epithelial cell ratio (PAR) in the adenoma-dysplasia-carcinoma sequence (ADCS), and to examine its association with osteopontin (OPN), a previously identified protein product related to cancer development. One mm diameter cores from 13 healthy colons, 13 adenomas and 13 colon carcinoma samples were included into a tissue microarray (TMA) block. TUNEL reaction and Ki-67 immunohistochemistry were applied to determine the PAR. The osteopontin protein was also immunodetected. Stained slides were semiquantitatively evaluated using digital microscope and statistically analyzed with logistic regression and Fisher's exact test. The PAR continuously increased along the ADCS. It was significantly (p

Original languageEnglish
Pages (from-to)541-545
Number of pages5
JournalPathology and Oncology Research
Volume16
Issue number4
DOIs
Publication statusPublished - Dec 2010

Fingerprint

Osteopontin
Adenoma
Carcinoma
Colon
In Situ Nick-End Labeling
Colorectal Neoplasms
Neoplasms
Proteins
Logistic Models
Epithelial Cells
Immunohistochemistry
Apoptosis

Keywords

  • Adenoma-dysplasia-carcinoma sequence
  • Gene expression
  • Proliferative/apoptotic ratio
  • Tissue microarray

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Elevated osteopontin expression and proliferative/apoptotic ratio in the colorectal adenoma-dysplasia-carcinoma sequence. / Valcz, Gábor; Sípos, F.; Krenács, T.; Molnár, Jeannette; Patai, Árpád V.; Leiszter, Katalin; Tóth, Kinga; Solymosi, N.; Galamb, O.; Molnár, B.; Tulassay, Z.

In: Pathology and Oncology Research, Vol. 16, No. 4, 12.2010, p. 541-545.

Research output: Contribution to journalArticle

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