Elevated miR-33a and miR-224 in steatotic chronic hepatitis C liver biopsies

Gabor Lendvai, K. Jármay, Gizella Karácsony, Tünde Halász, I. Kovalszky, Kornélia Baghy, T. Wittmann, Z. Schaff, András Kiss

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

AIM: To assess the expression of selected microRNAs (miRNA) in hepatitis C, steatotic hepatitis C, noninfected steatotic and normal liver tissues.

METHODS: The relative expression levels of miR-21, miR-33a, miR-96, miR-122, miR-125b, miR-221 and miR-224 were determined in 76 RNA samples isolated from 18 non-steatotic and 28 steatotic chronic hepatitis C (CHC and CHC-Steatosis, respectively) cases, 18 non-infected, steatotic liver biopsies of metabolic origin (Steatosis) and 12 normal formalin-fixed paraffin-embedded liver tissues using TaqMan MicroRNA Assays. All CHC biopsy samples were obtained prior to initiating therapy. Patients' serum biochemical values, which included glucose, triglyceride, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl-transferase (GGT), alkaline phosphatase (AP), were obtained and correlated with relative miRNA expression.

RESULTS: When compared with control non-infected liver samples, miR-122 and miR-221 levels were reduced in CHC-Steatosis (P <0.03) and in CHC, CHCSteatosis and Steatosis (P <0.01). Alternatively, the expression of miR-33a and miR-224 were elevated in CHC-Steatosis and Steatosis in comparison to control tissue (P <0.01). The levels of miR-33a and miR-224 in CHC-Steatosis (P <0.02) and miR-224 in Steatosis (P <0.001) were increased in comparison to CHC samples. By contrast, the expression of miR-21 did not differ statistically between diseased and normal liver samples. Levels of miR-33a correlated negatively with serum AST and AP levels in Steatosis as well as with necroinflammatory grade in CHC, whereas miR-21 correlated positively with AST in Steatosis and displayed negative correlation with triglyceride level in CHC-Steatosis. In contrast, miRNA levels were not correlated with ALT, GGT, cholesterol levels or fibrosis stage.

CONCLUSION: Differences in miRNA expression were observed between CHC and steatotic CHC, CHC and steatotic liver, but not between steatotic CHC and steatotic liver of metabolic origin.

Original languageEnglish
Pages (from-to)15343-15350
Number of pages8
JournalWorld Journal of Gastroenterology
Volume20
Issue number41
DOIs
Publication statusPublished - Nov 7 2014

Fingerprint

Chronic Hepatitis C
MicroRNAs
Biopsy
Liver
Aspartate Aminotransferases
Hepatitis C
Transferases
Alanine Transaminase
Alkaline Phosphatase
Triglycerides
Cholesterol
Serum
Paraffin
Formaldehyde
Liver Diseases
Fibrosis
RNA
Glucose

Keywords

  • Chronic hepatitis C
  • Expression
  • MicroRNA
  • MiR-224
  • MiR-33a
  • Steatosis

ASJC Scopus subject areas

  • Gastroenterology
  • Medicine(all)

Cite this

Elevated miR-33a and miR-224 in steatotic chronic hepatitis C liver biopsies. / Lendvai, Gabor; Jármay, K.; Karácsony, Gizella; Halász, Tünde; Kovalszky, I.; Baghy, Kornélia; Wittmann, T.; Schaff, Z.; Kiss, András.

In: World Journal of Gastroenterology, Vol. 20, No. 41, 07.11.2014, p. 15343-15350.

Research output: Contribution to journalArticle

Lendvai, Gabor ; Jármay, K. ; Karácsony, Gizella ; Halász, Tünde ; Kovalszky, I. ; Baghy, Kornélia ; Wittmann, T. ; Schaff, Z. ; Kiss, András. / Elevated miR-33a and miR-224 in steatotic chronic hepatitis C liver biopsies. In: World Journal of Gastroenterology. 2014 ; Vol. 20, No. 41. pp. 15343-15350.
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abstract = "AIM: To assess the expression of selected microRNAs (miRNA) in hepatitis C, steatotic hepatitis C, noninfected steatotic and normal liver tissues.METHODS: The relative expression levels of miR-21, miR-33a, miR-96, miR-122, miR-125b, miR-221 and miR-224 were determined in 76 RNA samples isolated from 18 non-steatotic and 28 steatotic chronic hepatitis C (CHC and CHC-Steatosis, respectively) cases, 18 non-infected, steatotic liver biopsies of metabolic origin (Steatosis) and 12 normal formalin-fixed paraffin-embedded liver tissues using TaqMan MicroRNA Assays. All CHC biopsy samples were obtained prior to initiating therapy. Patients' serum biochemical values, which included glucose, triglyceride, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl-transferase (GGT), alkaline phosphatase (AP), were obtained and correlated with relative miRNA expression.RESULTS: When compared with control non-infected liver samples, miR-122 and miR-221 levels were reduced in CHC-Steatosis (P <0.03) and in CHC, CHCSteatosis and Steatosis (P <0.01). Alternatively, the expression of miR-33a and miR-224 were elevated in CHC-Steatosis and Steatosis in comparison to control tissue (P <0.01). The levels of miR-33a and miR-224 in CHC-Steatosis (P <0.02) and miR-224 in Steatosis (P <0.001) were increased in comparison to CHC samples. By contrast, the expression of miR-21 did not differ statistically between diseased and normal liver samples. Levels of miR-33a correlated negatively with serum AST and AP levels in Steatosis as well as with necroinflammatory grade in CHC, whereas miR-21 correlated positively with AST in Steatosis and displayed negative correlation with triglyceride level in CHC-Steatosis. In contrast, miRNA levels were not correlated with ALT, GGT, cholesterol levels or fibrosis stage.CONCLUSION: Differences in miRNA expression were observed between CHC and steatotic CHC, CHC and steatotic liver, but not between steatotic CHC and steatotic liver of metabolic origin.",
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AU - Jármay, K.

AU - Karácsony, Gizella

AU - Halász, Tünde

AU - Kovalszky, I.

AU - Baghy, Kornélia

AU - Wittmann, T.

AU - Schaff, Z.

AU - Kiss, András

PY - 2014/11/7

Y1 - 2014/11/7

N2 - AIM: To assess the expression of selected microRNAs (miRNA) in hepatitis C, steatotic hepatitis C, noninfected steatotic and normal liver tissues.METHODS: The relative expression levels of miR-21, miR-33a, miR-96, miR-122, miR-125b, miR-221 and miR-224 were determined in 76 RNA samples isolated from 18 non-steatotic and 28 steatotic chronic hepatitis C (CHC and CHC-Steatosis, respectively) cases, 18 non-infected, steatotic liver biopsies of metabolic origin (Steatosis) and 12 normal formalin-fixed paraffin-embedded liver tissues using TaqMan MicroRNA Assays. All CHC biopsy samples were obtained prior to initiating therapy. Patients' serum biochemical values, which included glucose, triglyceride, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl-transferase (GGT), alkaline phosphatase (AP), were obtained and correlated with relative miRNA expression.RESULTS: When compared with control non-infected liver samples, miR-122 and miR-221 levels were reduced in CHC-Steatosis (P <0.03) and in CHC, CHCSteatosis and Steatosis (P <0.01). Alternatively, the expression of miR-33a and miR-224 were elevated in CHC-Steatosis and Steatosis in comparison to control tissue (P <0.01). The levels of miR-33a and miR-224 in CHC-Steatosis (P <0.02) and miR-224 in Steatosis (P <0.001) were increased in comparison to CHC samples. By contrast, the expression of miR-21 did not differ statistically between diseased and normal liver samples. Levels of miR-33a correlated negatively with serum AST and AP levels in Steatosis as well as with necroinflammatory grade in CHC, whereas miR-21 correlated positively with AST in Steatosis and displayed negative correlation with triglyceride level in CHC-Steatosis. In contrast, miRNA levels were not correlated with ALT, GGT, cholesterol levels or fibrosis stage.CONCLUSION: Differences in miRNA expression were observed between CHC and steatotic CHC, CHC and steatotic liver, but not between steatotic CHC and steatotic liver of metabolic origin.

AB - AIM: To assess the expression of selected microRNAs (miRNA) in hepatitis C, steatotic hepatitis C, noninfected steatotic and normal liver tissues.METHODS: The relative expression levels of miR-21, miR-33a, miR-96, miR-122, miR-125b, miR-221 and miR-224 were determined in 76 RNA samples isolated from 18 non-steatotic and 28 steatotic chronic hepatitis C (CHC and CHC-Steatosis, respectively) cases, 18 non-infected, steatotic liver biopsies of metabolic origin (Steatosis) and 12 normal formalin-fixed paraffin-embedded liver tissues using TaqMan MicroRNA Assays. All CHC biopsy samples were obtained prior to initiating therapy. Patients' serum biochemical values, which included glucose, triglyceride, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl-transferase (GGT), alkaline phosphatase (AP), were obtained and correlated with relative miRNA expression.RESULTS: When compared with control non-infected liver samples, miR-122 and miR-221 levels were reduced in CHC-Steatosis (P <0.03) and in CHC, CHCSteatosis and Steatosis (P <0.01). Alternatively, the expression of miR-33a and miR-224 were elevated in CHC-Steatosis and Steatosis in comparison to control tissue (P <0.01). The levels of miR-33a and miR-224 in CHC-Steatosis (P <0.02) and miR-224 in Steatosis (P <0.001) were increased in comparison to CHC samples. By contrast, the expression of miR-21 did not differ statistically between diseased and normal liver samples. Levels of miR-33a correlated negatively with serum AST and AP levels in Steatosis as well as with necroinflammatory grade in CHC, whereas miR-21 correlated positively with AST in Steatosis and displayed negative correlation with triglyceride level in CHC-Steatosis. In contrast, miRNA levels were not correlated with ALT, GGT, cholesterol levels or fibrosis stage.CONCLUSION: Differences in miRNA expression were observed between CHC and steatotic CHC, CHC and steatotic liver, but not between steatotic CHC and steatotic liver of metabolic origin.

KW - Chronic hepatitis C

KW - Expression

KW - MicroRNA

KW - MiR-224

KW - MiR-33a

KW - Steatosis

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