Elevated Levels of Antibodies Against 70 kDa Heat Shock Proteins in the Sera of Patients With HIV Infection

Judit Kocsis, Z. Prohászka, Adrienn Biró, G. Füst, D. Bánhegyi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Heat shock proteins (Hsp), especially 70 kDa heat shock protein (Hsp70) play an important role in the life cycle of HIV-1 virus. Hsp70 is over-expressed in HIV-infected cells and this is the most abundant Hsp associated with HIV virions. The aim of our study was to investigate whether HIV infection increases the extent of specific humoral immune response against Hsp70. The serum concentration of anti-Hsp70 IgG antibodies was measured in 47 HIV-infected patients, and 62 healthy, HIV-seronegative persons. Nineteen patients on highly active anti-retroviral therapy (HAART) were followed for 24 months in a longitudinal study. Anti-Hsp70 antibodies were measured by ELISA, using recombinant human Hsp70. Levels of anti-Hsp70 antibodies were significantly (P <0.0001) higher in the HIV-infected patients (median: 1409 (25th-75th percentile: 1031-2214) AU/ml) than in healthy control subjects (626 (429-970) AU/ml). In 19 HIV patients, serum levels of anti-Hsp70 antibodies significantly (P <0.001) decreased during 24 (11 - 41) months HAART (1309 (887-2213) AU/ml before and 640 (386-959) AU/ml during HAART), accompanied by viral load reduction and CD4+ count elevation. It is concluded that HIV-infection induces a marked increase in the anti-Hsp70 antibody levels, which is consistent with the enhanced expression of Hsp70 on the surface of HIV-infected cells and/or incorporation of the protein into the membrane of HIV virions.

Original languageEnglish
Pages (from-to)480-482
Number of pages3
JournalJournal of Medical Virology
Volume71
Issue number4
DOIs
Publication statusPublished - Dec 2003

Fingerprint

HSP70 Heat-Shock Proteins
HIV Infections
HIV
Antibodies
Serum
Anti-Idiotypic Antibodies
Heat-Shock Proteins
Virion
CD4 Lymphocyte Count
Humoral Immunity
Life Cycle Stages
Viral Load
Longitudinal Studies
HIV-1
Healthy Volunteers
Membrane Proteins
Therapeutics
Enzyme-Linked Immunosorbent Assay
Viruses

Keywords

  • Anti-Hsp70 antibodies, HIV
  • HAART
  • Heat shock proteins
  • Hsp70

ASJC Scopus subject areas

  • Virology

Cite this

Elevated Levels of Antibodies Against 70 kDa Heat Shock Proteins in the Sera of Patients With HIV Infection. / Kocsis, Judit; Prohászka, Z.; Biró, Adrienn; Füst, G.; Bánhegyi, D.

In: Journal of Medical Virology, Vol. 71, No. 4, 12.2003, p. 480-482.

Research output: Contribution to journalArticle

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