Elevated CREB activity in embryonic fibroblasts of gene-targeted histamine deficient mice

K. Hegyi, A. Falus, S. Toth

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Objectives: Histamine is a known inducer of cAMP-responsive element binding protein (CREB), which plays a key role in initiation of adipogenesis. Our present goal was to study how histamine deficiency impacts CREB signalling and adipogenesis. Methods: We used a histidine-decarboxylase gene-targeted (HDC KO) mice model lacking endogenous histamine. We measured CREB activity and expression by EMSA, Western blot and real-time RT-PCR, as well as cAMP levels by ELISA in primary embryonic fibroblasts derived from WT and HDC KO mice. The ability of these cells to form adipocytes was also tested in preliminary experiments. Results: We found that in the absence of the histamine, cells show higher constitutive CREB activity and greatly increased intracellular cAMP levels, as well as that in contrast to WT cells, HDC KO fibroblasts are more prone to differentiate into adipocytes. Conclusion: These data suggest a newly recognised inhibitory role for histamine in CREB activity and draws attention to the potential role of histamine in adipocyte differentiation.

Original languageEnglish
Pages (from-to)339-344
Number of pages6
JournalInflammation Research
Volume56
Issue number8
DOIs
Publication statusPublished - Aug 1 2007

Keywords

  • Adipogenesis
  • CREB
  • Histamine
  • Histidine-decarboxylase
  • cAMP

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Fingerprint Dive into the research topics of 'Elevated CREB activity in embryonic fibroblasts of gene-targeted histamine deficient mice'. Together they form a unique fingerprint.

  • Cite this