Elevated complement factor H levels in asthmatic sputa

Zsóka Weiszhár, András Bikov, Gabriella Gálffy, Lilla Tamási, Ildikó Ungvári, Csaba Szalai, György Losonczy, Ildikó Horváth

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: The alternative pathway of the complement system is known to play a role in the generation of asthmatic airway inflammation, but its regulatory complement protein, factor H has not been investigated in this disease. Purpose Our aim was to determine the local bronchial complement factor H (CFH) levels in asthma, and to investigate its relationship with complement activation, systemic CFH concentrations and clinical characteristics of patients. Methods: Induced sputum and plasma were collected from 21 healthy and 26 asthmatic subjects, and complement factor H and SC5b-9 concentrations were assessed by ELISA. Total protein concentrations were determined by biuret-reaction based microassay system from induced sputa. Results: CFH was detectable in 81 % of healthy and 100 % of asthmatic subjects, while SC5b-9 exceeded the detection limit in 62 % of healthy subjects and 85 % of asthmatic patients. Sputum CFH concentrations and CFH/protein ratios were increased in samples from asthmatic patients, and correlated with loss of lung function, asthma control, severity and medication intensity, but not with plasma CFH concentrations. Sputum CFH/protein ratios were in positive correlation also with sputum eosinophilic cell counts in asthma. SC5b-9 concentrations were not higher in the asthmatic sputa, although they correlated with sputum CFH concentrations. Conclusions: CFH level is elevated on asthmatic airway surface, and may be associated with uncontrolled inflammation in asthma.

Original languageEnglish
Pages (from-to)496-505
Number of pages10
JournalJournal of Clinical Immunology
Volume33
Issue number2
DOIs
Publication statusPublished - Jan 1 2013

Keywords

  • Airway inflammation
  • Asthma control
  • Complement factor H
  • Induced sputum
  • Severe asthma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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