Elevated complement C3 is associated with early restenosis after eversion carotid endarterectomy

G. Széplaki, L. Varga, Judit Laki, Edit Dósa, Hans O. Madsen, Z. Prohászka, Attila Szabó, G. Acsády, L. Selmeci, Peter Garred, G. Füst, L. Entz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Early restenosis following carotid endarterectomy (CEA) is an onflammatory process leading to myointimal hyperplasia of smooth muscle cells. The risk for restenosis is increased in homozygous carriers of the normal (A) allele of mannose-binding lectin (MBL2) gene. Our objective was to study the associations of C3 and as control three non-complement acute-phase reactants (APRs) (C-reactive protein, haptoglobin and α2HS-glycoprotein) with early restenosis following CEA. We also considered, whether MBL2 genotype relates to C3 levels and to the risk of restenosis. Concentrations of the APRs were determined by radial immunodiffusion or immunoturbidimetric methods in 64 patients who underwent eversion CEA and were followed up with carotid duplex scan (CDS) examinations for at least one year. MBL2 genotypes were determined by a PCR-SSP method. C3 levels increased during the follow-up and correlated with the percentage of restenosis detected by CDS at 14 months post-surgery, in MBL2 A/A allele carriers. Patients with high C3 levels had nearly five-fold higher odds for the presence of significant restenosis (>50% reduction in diameter) even after adjusting for MBL2 genotype, age and gender. By contrast, no such associations were detected between the non-complement APRs and early restenosis. C3 is associated with and might have a direct role in the development of an early restenosis following CEA, which is partially related to an intact MBL lectin pathway, thus determining C3 levels might have clinical importance. On the other hand, our results indicate that the regulation of C3 differs from non-complement APRs.

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalThrombosis and Haemostasis
Volume96
Issue number4
DOIs
Publication statusPublished - Oct 2006

Fingerprint

Complement C3
Acute-Phase Proteins
Carotid Endarterectomy
Genotype
Alleles
Mannose-Binding Lectin
Haptoglobins
Immunodiffusion
Lectins
C-Reactive Protein
Smooth Muscle Myocytes
Hyperplasia
Glycoproteins
Polymerase Chain Reaction
Genes

Keywords

  • Carotid arteries
  • Complement C3
  • Endarterectomy
  • Mannose binding lectin
  • Restenosis

ASJC Scopus subject areas

  • Hematology

Cite this

Elevated complement C3 is associated with early restenosis after eversion carotid endarterectomy. / Széplaki, G.; Varga, L.; Laki, Judit; Dósa, Edit; Madsen, Hans O.; Prohászka, Z.; Szabó, Attila; Acsády, G.; Selmeci, L.; Garred, Peter; Füst, G.; Entz, L.

In: Thrombosis and Haemostasis, Vol. 96, No. 4, 10.2006, p. 529-534.

Research output: Contribution to journalArticle

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abstract = "Early restenosis following carotid endarterectomy (CEA) is an onflammatory process leading to myointimal hyperplasia of smooth muscle cells. The risk for restenosis is increased in homozygous carriers of the normal (A) allele of mannose-binding lectin (MBL2) gene. Our objective was to study the associations of C3 and as control three non-complement acute-phase reactants (APRs) (C-reactive protein, haptoglobin and α2HS-glycoprotein) with early restenosis following CEA. We also considered, whether MBL2 genotype relates to C3 levels and to the risk of restenosis. Concentrations of the APRs were determined by radial immunodiffusion or immunoturbidimetric methods in 64 patients who underwent eversion CEA and were followed up with carotid duplex scan (CDS) examinations for at least one year. MBL2 genotypes were determined by a PCR-SSP method. C3 levels increased during the follow-up and correlated with the percentage of restenosis detected by CDS at 14 months post-surgery, in MBL2 A/A allele carriers. Patients with high C3 levels had nearly five-fold higher odds for the presence of significant restenosis (>50{\%} reduction in diameter) even after adjusting for MBL2 genotype, age and gender. By contrast, no such associations were detected between the non-complement APRs and early restenosis. C3 is associated with and might have a direct role in the development of an early restenosis following CEA, which is partially related to an intact MBL lectin pathway, thus determining C3 levels might have clinical importance. On the other hand, our results indicate that the regulation of C3 differs from non-complement APRs.",
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