Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods

Björn De Samber, Geert Silversmit, Karel De Schamphelaere, Roel Evens, Tom Schoonjans, Bart Vekemans, Colin Janssen, Bert Masschaele, Luc Van Hoorebeke, Imre Szalóki, Frank Vanhaecke, Karen Rickers, Gerald Falkenberg, Laszlo Vincze

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Abstract

Synchrotron radiation based confocal micro-XRF was employed to unravel the tissue-specific three-dimensional (3D) distribution of metals down to trace concentration levels in a non-destructive manner within the crustacean Daphnia magna, an ecotoxicological model organism. Next to the analytical characterization of the employed confocal micro-XRF set-up, specific areas of metal accumulation in different cross-sections of interest within the organism were investigated. The use of a fast dynamic (continuous) scanning approach delivered 3D information on major, minor and trace element distributions in specific sub-regions within Daphnia magna. Coupling the obtained elemental information with microscopic morphological data obtained by laboratory absorption microtomography, full 3D element-to-tissue correlation could be derived, allowing a more detailed interpretation of the obtained results with respect to metal accumulation within this model organism.

Original languageEnglish
Pages (from-to)544-553
Number of pages10
JournalJournal of analytical atomic spectrometry
Volume25
Issue number4
DOIs
Publication statusPublished - Apr 6 2010

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

De Samber, B., Silversmit, G., De Schamphelaere, K., Evens, R., Schoonjans, T., Vekemans, B., Janssen, C., Masschaele, B., Van Hoorebeke, L., Szalóki, I., Vanhaecke, F., Rickers, K., Falkenberg, G., & Vincze, L. (2010). Element-to-tissue correlation in biological samples determined by three-dimensional X-ray imaging methods. Journal of analytical atomic spectrometry, 25(4), 544-553. https://doi.org/10.1039/b918624g