Electrosynthesized molecularly imprinted polyscopoletin nanofilms for human serum albumin detection

Zorica Stojanovic, Júlia Erdőssy, Katalin Keltai, Frieder W. Scheller, R. Gyurcsányi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Molecularly imprinted polymers (MIPs) rendered selective solely by the imprinting with protein templates lacking of distinctive properties to facilitate strong target-MIP interaction are likely to exhibit medium to low template binding affinities. While this prohibits the use of such MIPs for applications requiring the assessment of very low template concentrations, their implementation for the quantification of high-abundance proteins seems to have a clear niche in the analytical practice. We investigated this opportunity by developing a polyscopoletin-based MIP nanofilm for the electrochemical determination of elevated human serum albumin (HSA) in urine. As reference for a low abundance protein ferritin-MIPs were also prepared by the same procedure. Under optimal conditions, the imprinted sensors gave a linear response to HSA in the concentration range of 20–100 mg/dm3, and to ferritin in the range of 120–360 mg/dm3. While as expected the obtained limit of detection was not sufficient to determine endogenous ferritin in plasma, the HSA-sensor was successfully employed to analyse urine samples of patients with albuminuria. The results suggest that MIP-based sensors may be applicable for quantifying high abundance proteins in a clinical setting.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalAnalytica Chimica Acta
Volume977
DOIs
Publication statusPublished - Jul 18 2017

Keywords

  • Ferritin
  • Human serum albumin
  • Molecularly imprinted polymer
  • Scopoletin
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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