Electrospray tandem mass spectrometric analysis of 24 amino acids from human blood spot without chemical derivatization used for neonatal screening

Kornél Nagy, Zoltán Takáts, Károly Vékey

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A new sample preparation method based on solid phase extraction without classical chemical derivatization, coupled with Multiple Reaction Monitoring allowing sensitive and simultaneous detection of more than 100 chemically different metabolites from one injection, was discussed. PE-AX (Pre Equilibrated Strong Anion Exchange) cartridges were used to perform anion exchange solid phase extraction after the aqueous extraction of the blood spot. Assay statistics, recovery data were obtained and neonate suffering from hyperprolinemia was identified. The recovered data and the reproducible MRM amino acid profile of the blood sample proved the applicability of the method for the screening of metabolic disorders and distinguishing normal and pathologic samples.

Original languageEnglish
Title of host publicationProceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages553
Number of pages1
Publication statusPublished - 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

Fingerprint

Screening
Blood
Amino Acids
Anions
Metabolites
Assays
Ion exchange
Statistics
Recovery
Monitoring

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Nagy, K., Takáts, Z., & Vékey, K. (2002). Electrospray tandem mass spectrometric analysis of 24 amino acids from human blood spot without chemical derivatization used for neonatal screening. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 553)

Electrospray tandem mass spectrometric analysis of 24 amino acids from human blood spot without chemical derivatization used for neonatal screening. / Nagy, Kornél; Takáts, Zoltán; Vékey, Károly.

Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 553.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nagy, K, Takáts, Z & Vékey, K 2002, Electrospray tandem mass spectrometric analysis of 24 amino acids from human blood spot without chemical derivatization used for neonatal screening. in Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 553, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.
Nagy K, Takáts Z, Vékey K. Electrospray tandem mass spectrometric analysis of 24 amino acids from human blood spot without chemical derivatization used for neonatal screening. In Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 553
Nagy, Kornél ; Takáts, Zoltán ; Vékey, Károly. / Electrospray tandem mass spectrometric analysis of 24 amino acids from human blood spot without chemical derivatization used for neonatal screening. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. pp. 553
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