Electrospray ionization fourier transform ion cyclotron resonance mass spectrometry of human α-1-acid glycoprotein

Kornél Nagy, Károly Vékey, Tímea Imre, Krisztina Ludányi, Mark P. Barrow, Peter J. Derrick

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The ultrahigh resolution and sensitivity of electrospray ionization Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry have for the first time been exploited for the characterization of highly sialylated glycoproteins, using human α-1-acid glycoprotein as the model compound. An alternative approach to the widely used high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization (MALDI) assays is described. This new method does not require any enzymatic or chemical digestion (removal of sialyl groups or deglycosylation), chemical derivatization (introduction of chromophore groups), or preliminary chromatographic separation (HPLC or electrophoresis). Following ESI and accumulation of ions in a hexapole ion guide, ions are injected into the ICR cell. A selected mass window from the overall ion population is isolated and axialized prior to detection. After acquisition and Fourier transform of the transient signal the resulted spectrum is evaluated in order to determine the charge state of the detected ions and the isotope pattern of the measured protein glycoform. The presence of ions from the same glycoform with different charge states was confirmed. The advantages and limitations of the technique are discussed. Future prospects and possible applications are indicated.

Original languageEnglish
Pages (from-to)4998-5005
Number of pages8
JournalAnalytical Chemistry
Volume76
Issue number17
DOIs
Publication statusPublished - Sep 1 2004

ASJC Scopus subject areas

  • Analytical Chemistry

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