Electroporation-delivered transdermal neostigmine in rats: Equivalent action to intravenous administration

Szilvia Berkó, Kálmán F. Szűcs, Boglárka Balázs, Erzsébet Csányi, Gábor Varju, Anita Sztojkov-Ivanov, Mária Budai-Szűcs, Judit Bóta, R. Gáspár

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: Transdermal electroporation has become one of the most promising noninvasive methods for drug administration, with greatly increased transport of macromolecules through the skin. The cecal-contracting effects of repeated transdermal electroporation delivery and intravenous administration of neostigmine were compared in anesthetized rats. Methods: The cecal contractions were detected with implantable strain gauge sensors, and the plasma levels of neostigmine were followed by high-performance liquid chromatography. Results: Both intravenously and EP-administered neostigmine (0.2–66.7 μg/kg) increased the cecal contractions in a dose-dependent manner. For both the low doses and the highest dose, the neostigmine plasma concentrations were the same after the two modes of administration, while an insignificantly higher level was observed at a dose of 20 μg/kg after intravenous administration as compared with the electroporation route. The contractile responses did not differ significantly after the two administration routes. Conclusion: The results suggest that electroporation-delivered neostigmine elicits action equivalent to that observed after intravenous administration as concerning both time and intensity. Electroporation permits the delivery of even lower doses of water-soluble compounds through the skin, which is very promising for clinical practice.

Original languageEnglish
Pages (from-to)1695-1701
Number of pages7
JournalDrug Design, Development and Therapy
Volume10
DOIs
Publication statusPublished - May 19 2016

Fingerprint

Neostigmine
Electroporation
Intravenous Administration
Skin
High Pressure Liquid Chromatography
Water
Pharmaceutical Preparations

Keywords

  • Contraction
  • Electroporation
  • Neostigmine
  • Plasma level
  • Smooth muscle
  • Transdermal

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery

Cite this

Electroporation-delivered transdermal neostigmine in rats : Equivalent action to intravenous administration. / Berkó, Szilvia; Szűcs, Kálmán F.; Balázs, Boglárka; Csányi, Erzsébet; Varju, Gábor; Sztojkov-Ivanov, Anita; Budai-Szűcs, Mária; Bóta, Judit; Gáspár, R.

In: Drug Design, Development and Therapy, Vol. 10, 19.05.2016, p. 1695-1701.

Research output: Contribution to journalArticle

Berkó, S, Szűcs, KF, Balázs, B, Csányi, E, Varju, G, Sztojkov-Ivanov, A, Budai-Szűcs, M, Bóta, J & Gáspár, R 2016, 'Electroporation-delivered transdermal neostigmine in rats: Equivalent action to intravenous administration', Drug Design, Development and Therapy, vol. 10, pp. 1695-1701. https://doi.org/10.2147/DDDT.S102959
Berkó, Szilvia ; Szűcs, Kálmán F. ; Balázs, Boglárka ; Csányi, Erzsébet ; Varju, Gábor ; Sztojkov-Ivanov, Anita ; Budai-Szűcs, Mária ; Bóta, Judit ; Gáspár, R. / Electroporation-delivered transdermal neostigmine in rats : Equivalent action to intravenous administration. In: Drug Design, Development and Therapy. 2016 ; Vol. 10. pp. 1695-1701.
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