Electrophysiological effects of adenosine and adenosine triphosphate on sheep Purkinje fibres under normal and simulated ischaemic conditions

G. Boachie-Ansah, K. A. Kane, J. Parratt

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The electrophysiological effects of adenosine and adenosine triphosphate (ATP) were examined in sheep Purkinje fibres, superfused in vitro with either a normal or a hypoxic, hyperkalaemic and acidotic physiological salt solution (PSS). The ability of adenosine to modify the effects of noradrenaline on action potential characteristics was also investigated. The only statistically significant effects of adenosine (10-6 - 10-4 M) and of ATP (10-6 - 10-4 M) on normal action potential characteristics were a slight dose-dependent shortening of the action potential by adenosine and a depolarization by ATP, 10-4 M. Superfusion with a hypoxic, hyperkalaemic and acidotic PSS caused marked reductions in resting membrane potential, upstroke and duration of the action potential. Both adenosine and ATP attenuated the reduction in the rate of rise of the upstroke and the amplitude of the action potential caused by the modified PSS. Adenosine did not alter the noradrenaline-induced effects on automaticity or on action potentials of normal or depressed Purkinje fibres. Adenosine and ATP had electrophysiological effects on Purkinje fibres, exposed to conditions in vitro that mimic mild myocardial ischaemia, that were different from those observed on normally polarized fibres.

Original languageEnglish
Pages (from-to)240-246
Number of pages7
JournalBritish Journal of Pharmacology
Volume97
Issue number1
Publication statusPublished - 1989

Fingerprint

Purkinje Fibers
Adenosine
Sheep
Action Potentials
Adenosine Triphosphate
Salts
Norepinephrine
Membrane Potentials
Myocardial Ischemia

ASJC Scopus subject areas

  • Pharmacology

Cite this

Electrophysiological effects of adenosine and adenosine triphosphate on sheep Purkinje fibres under normal and simulated ischaemic conditions. / Boachie-Ansah, G.; Kane, K. A.; Parratt, J.

In: British Journal of Pharmacology, Vol. 97, No. 1, 1989, p. 240-246.

Research output: Contribution to journalArticle

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