Electrophysiological characterization of neural stem/progenitor cells during in vitro differentiation

Study with an immortalized neuroectodermal cell line

Marta Jelitai, M. Anderová, A. Chvátal, E. Madarász

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Despite the accumulating data on the molecular and cell biological characteristics of neural stem/progenitor cells, their electrophysiological properties are not well understood. In the present work, changes in the membrane properties and current profiles were investigated in the course of in vitro-induced neuron formation in NE-4C cells. Induction by retinoic acid resulted in neuronal differentiation of about 50% of cells. Voltage-dependent Na+ currents appeared early in neuronal commitment, often preceding any morphological changes. A-type K+ currents were detected only at the stage of network formation by neuronal processes. Flat, epithelial-like, nestin-expressing progenitors persisted beside differentiated neurons and astrocytes. Stem/progenitor cells were gap junction coupled and displayed large, symmetrical, voltage-independent currents. By the blocking of gap junction communication, voltage-independent conductance was significantly reduced, and delayed-rectifying K+ currents became detectable. Our data indicate that voltage-independent symmetrical currents and gap junction coupling are characteristic physiological features of neural stem and progenitor cells regardless of the developmental state of their cellular environment.

Original languageEnglish
Pages (from-to)1606-1617
Number of pages12
JournalJournal of Neuroscience Research
Volume85
Issue number8
DOIs
Publication statusPublished - Jun 2007

Fingerprint

Neural Stem Cells
Gap Junctions
Stem Cells
Cell Line
Neurons
Nestin
Tretinoin
Astrocytes
Membranes
In Vitro Techniques

Keywords

  • Gap junction coupling
  • Neural stem/progenitor cells
  • Neuronal commitment
  • Passive current

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Electrophysiological characterization of neural stem/progenitor cells during in vitro differentiation : Study with an immortalized neuroectodermal cell line. / Jelitai, Marta; Anderová, M.; Chvátal, A.; Madarász, E.

In: Journal of Neuroscience Research, Vol. 85, No. 8, 06.2007, p. 1606-1617.

Research output: Contribution to journalArticle

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