Contour clamped homogeneous electric field (CHEF) gel electrophoresis was used to obtain electrophoretic karyotypes from nine Mucor strains representing five different species (M. bainieri, M. circinelloides, M. mucedo, M. plumbeus and M. racemosus). The chromosomal banding patterns revealed high variability among the isolates. The sizes of the DNA in the Mucor chromosomes were estimated to be between 2.5 and 8.7 Mb. The total genome sizes were calculated to be between 30.0 and 44.7 Mb. The applicability of these electrophoretic karyotypes for the investigation of genome structure, for strain identification and for species delimitation is considered.
|Number of pages||5|
|Journal||Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology|
|Publication status||Published - Jan 1 2000|
- Chromosome-length polymorphism
- Electrophoretic karyotyping
ASJC Scopus subject areas
- Molecular Biology