Electrophoretic karyotypes of Phaffia rhodozyma strains

Ágnes Nagy, Nándor Garamszegi, C. Vágvölgyi, L. Ferenczy

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Electrophoretic karyotypes of strains from the astaxanthin-producing yeast Phaffia rhodozyma have been established. Intact chromosomal DNA molecules released from protoplasts were separated by orthogonal field alternation gel electrophoresis (OFAGE) and contour clamped homogeneous electric field (CHEF). Both small and large chromosomal DNA molecules were resolved simultaneously by optimizing the running conditions. Electrophoretic karyotypes among the Phaffia isolates examined differed significantly. Seven to thirteen chromosomal bands, ranging in size from 0.83 Mb to 3.50 Mb, were resolved, giving total genome sizes of about 15.4 to 23.2 Mb. Ribosomal DNA has been assigned to chromosomal bands using a heterologous gene probe.

Original languageEnglish
Pages (from-to)315-318
Number of pages4
JournalFEMS Microbiology Letters
Volume123
Issue number3
Publication statusPublished - Nov 1 1994

Fingerprint

Karyotype
Genome Size
Protoplasts
Pulsed Field Gel Electrophoresis
DNA
Ribosomal DNA
Yeasts
Genes
astaxanthine

Keywords

  • Clamped homogeneous electric field
  • Electrophoretic karyotype
  • Gene localization
  • Orthogonal field alternation gel electrophoresis
  • Phaffia rhodozyma

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Electrophoretic karyotypes of Phaffia rhodozyma strains. / Nagy, Ágnes; Garamszegi, Nándor; Vágvölgyi, C.; Ferenczy, L.

In: FEMS Microbiology Letters, Vol. 123, No. 3, 01.11.1994, p. 315-318.

Research output: Contribution to journalArticle

Nagy, Ágnes ; Garamszegi, Nándor ; Vágvölgyi, C. ; Ferenczy, L. / Electrophoretic karyotypes of Phaffia rhodozyma strains. In: FEMS Microbiology Letters. 1994 ; Vol. 123, No. 3. pp. 315-318.
@article{b66c922680db4834b8841352f33198e1,
title = "Electrophoretic karyotypes of Phaffia rhodozyma strains",
abstract = "Electrophoretic karyotypes of strains from the astaxanthin-producing yeast Phaffia rhodozyma have been established. Intact chromosomal DNA molecules released from protoplasts were separated by orthogonal field alternation gel electrophoresis (OFAGE) and contour clamped homogeneous electric field (CHEF). Both small and large chromosomal DNA molecules were resolved simultaneously by optimizing the running conditions. Electrophoretic karyotypes among the Phaffia isolates examined differed significantly. Seven to thirteen chromosomal bands, ranging in size from 0.83 Mb to 3.50 Mb, were resolved, giving total genome sizes of about 15.4 to 23.2 Mb. Ribosomal DNA has been assigned to chromosomal bands using a heterologous gene probe.",
keywords = "Clamped homogeneous electric field, Electrophoretic karyotype, Gene localization, Orthogonal field alternation gel electrophoresis, Phaffia rhodozyma",
author = "{\'A}gnes Nagy and N{\'a}ndor Garamszegi and C. V{\'a}gv{\"o}lgyi and L. Ferenczy",
year = "1994",
month = "11",
day = "1",
language = "English",
volume = "123",
pages = "315--318",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Electrophoretic karyotypes of Phaffia rhodozyma strains

AU - Nagy, Ágnes

AU - Garamszegi, Nándor

AU - Vágvölgyi, C.

AU - Ferenczy, L.

PY - 1994/11/1

Y1 - 1994/11/1

N2 - Electrophoretic karyotypes of strains from the astaxanthin-producing yeast Phaffia rhodozyma have been established. Intact chromosomal DNA molecules released from protoplasts were separated by orthogonal field alternation gel electrophoresis (OFAGE) and contour clamped homogeneous electric field (CHEF). Both small and large chromosomal DNA molecules were resolved simultaneously by optimizing the running conditions. Electrophoretic karyotypes among the Phaffia isolates examined differed significantly. Seven to thirteen chromosomal bands, ranging in size from 0.83 Mb to 3.50 Mb, were resolved, giving total genome sizes of about 15.4 to 23.2 Mb. Ribosomal DNA has been assigned to chromosomal bands using a heterologous gene probe.

AB - Electrophoretic karyotypes of strains from the astaxanthin-producing yeast Phaffia rhodozyma have been established. Intact chromosomal DNA molecules released from protoplasts were separated by orthogonal field alternation gel electrophoresis (OFAGE) and contour clamped homogeneous electric field (CHEF). Both small and large chromosomal DNA molecules were resolved simultaneously by optimizing the running conditions. Electrophoretic karyotypes among the Phaffia isolates examined differed significantly. Seven to thirteen chromosomal bands, ranging in size from 0.83 Mb to 3.50 Mb, were resolved, giving total genome sizes of about 15.4 to 23.2 Mb. Ribosomal DNA has been assigned to chromosomal bands using a heterologous gene probe.

KW - Clamped homogeneous electric field

KW - Electrophoretic karyotype

KW - Gene localization

KW - Orthogonal field alternation gel electrophoresis

KW - Phaffia rhodozyma

UR - http://www.scopus.com/inward/record.url?scp=0027993112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027993112&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0027993112

VL - 123

SP - 315

EP - 318

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 3

ER -