Electrophoretic karyotypes of Phaffia rhodozyma strains

Ágnes Nagy, Nándor Garamszegi, Csaba Vágvölgyi, Lajos Ferenczy

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Abstract

Electrophoretic karyotypes of strains from the astaxanthin-producing yeast Phaffia rhodozyma have been established. Intact chromosomal DNA molecules released from protoplasts were separated by orthogonal field alternation gel electrophoresis (OFAGE) and contour clamped homogeneous electric field (CHEF). Both small and large chromosomal DNA molecules were resolved simultaneously by optimizing the running conditions. Electrophoretic karyotypes among the Phaffia isolates examined differed significantly. Seven to thirteen chromosomal bands, ranging in size from 0.83 Mb to 3.50 Mb, were resolved, giving total genome sizes of about 15.4 to 23.2 Mb. Ribosomal DNA has been assigned to chromosomal bands using a heterologous gene probe.

Original languageEnglish
Pages (from-to)315-318
Number of pages4
JournalFEMS microbiology letters
Volume123
Issue number3
DOIs
Publication statusPublished - Nov 1 1994

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Keywords

  • Clamped homogeneous electric field
  • Electrophoretic karyotype
  • Gene localization
  • Orthogonal field alternation gel electrophoresis
  • Phaffia rhodozyma

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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