Electrophoretic karyotypes and gene mapping in eight species of the Fusarium sections Arthrosporiella and Sporotrichiella

Csaba Fekete, Richárd Nagy, Alfons J.M. Debets, László Hornok

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Pulsed-field gel electrophoresis was used to identify karyotypes for eight species of the Fusarium sections Arthrosporiella and Sporotrichiella. The total number of chromosome-sized DNA molecules varied from six to nine, depending on the species. The sizes of chromosomes ranged from 0.4 to approximately 6.5 Mb which gave estimates of genome size of between 27.0 and 29.9 Mb. When fractionated chromosomes of the eight species were probed with Tox5, a gene coding for the key-enzyme of trichothecene biosynthesis, strong hybridization signals developed in F. poae and F. sporotrichioides, suggesting that of the eight species examined only these two have the genetic potentiality to produce trichothecene mycotoxins. By using heterologous probes from Aspergillus different rRNA loci have also been mapped on Fusarium chromosomes.

Original languageEnglish
Pages (from-to)500-504
Number of pages5
JournalCurrent Genetics
Volume24
Issue number6
DOIs
Publication statusPublished - Dec 1 1993

Keywords

  • Electrophoretic karyotyping
  • Fusarium
  • Gene mapping
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Genetics

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