Electrophoretic karyotype of Mucor circinelloides

A. Nagy, Cs Vágvölgyi, E. Balla, L. Ferenczy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Contour-clamped homogeneous electric field (CHEF) gel electrophoresis was used to separate chromosomal size DNA molecules of two Mucor circinelloides strains. Electrophoretic karyotypes revealed the presence of eight distinct bands for the M. circinelloides f. lusitanicus strain, and four, presumably multiple, bands for the M. circinelloides f. gryseo-cyanus strain. The approximate sizes of the resolved chromosomal DNA bands ranged from 2.3 to 8.1 Mb, giving estimated genome sizes of 38.7 and 32.6 Mb, respectively. Hybridisation techniques were used to assign the leuA gene to a chromosome.

Original languageEnglish
Pages (from-to)45-48
Number of pages4
JournalCurrent Genetics
Volume26
Issue number1
DOIs
Publication statusPublished - Jul 1 1994

    Fingerprint

Keywords

  • CHEF
  • Electrophoretic karyotype
  • Gene mapping
  • Mucor circinelloides

ASJC Scopus subject areas

  • Genetics

Cite this