Electrophoresis microchips for DNA analysis

Shaorong Liu, András Guttman

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Microfabricated bioanalytical devices are very efficient platforms for simultaneous analysis of biologically important molecules; they offer great potential for sensitive and large-scale DNA analysis. Indeed, electrophoresis-based sizing of nucleic acids is a leading application in microscale analysis. Adopting the concepts of conventional capillary electrophoresis, and various sample-preparation methods to microchip format, in conjunction with integrating the different analysis steps into a single monolithic system has opened up new levels in performance, functionality and throughput. Microfabricated bioanalytical devices, frequently referred to as "lab-on-a-chip", typically comprise microchannel networks, miniaturized polymerase chain reaction chambers and/or restriction digestion reactors, microseparation units and possible combinations of the above to accommodate DNA analysis. Some of the main advantages of miniaturization include improved performance, separation speed and throughput, reduced costs and reagent consumption, as well as integrated and parallel analysis for high-throughput environments. This review summarizes the recent advances in the field of microfabricated separation devices for genomics applications; it also addresses system integration and micropreparative functionalities.

Original languageEnglish
Pages (from-to)422-431
Number of pages10
JournalTrAC - Trends in Analytical Chemistry
Volume23
Issue number6
DOIs
Publication statusPublished - May 2004

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Keywords

  • DNA analysis
  • Electrophoresis
  • Microchip
  • System integration

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

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