The substrate of hydroxy-indol-O-methyl transferase (HIOMT) is N-acetylserotonin, which is synthesized by serotonin acetyltransferase. To demonstrate the localization of this enzyme in the pineal body, the uranyl or copper ferrocyanide technique was used. In both cases it appeared that the localization was of the mitochondrial type; in its (inner and outer) membranes as well as in its inner space. The reaction takes place in the presence of acetyl-Co-A. The copper ferrocyanide technique seems to have advantages as compared to the uranylferrocyanide method. Extramitochondrial precipitates or specific reactions were rare.
|Number of pages||3|
|Journal||Morphologiai es Igazsagugyi Orvosi Szemle|
|Publication status||Published - Jan 1 1976|
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