Electron microscopic immunocytochemical evidence for the existence of bidirectional synaptic connections between growth hormone-releasing hormone- and somatostatin-containing neurons in the hypothalamus of the rat

Sándor Horváth, M. Palkóvits, T. Görcs, Akira Arimura

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Synaptic contacts between growth hormone-releasing hormone (GHRH)- and somatostatin-containing neurons were demonstrated in the rat hypothalamus by a double-staining immunocytochemical method at the electron microscopic level. Somatostatin-immunoreactive nerve terminals synapse on GHRH-positive dendrites and cell bodies in the arcuate nucleus. A fine network of GHRH-immunopositive nerve terminals was observed at the light microscopic level in the rostral part of the periventricular nucleus and in the dorsal part of the arcuate nucleus around somatostatin-containing neuronal elements. With the electron microscopic synaptic contact between GHRH-containing nerve terminals and somatostatin-containing dendrites are demonstrated. The reciprocal innervation between GHRH- and somatostatin-containing neurons that project to the median eminence and regulate growth hormone secretion must allow them to coordiante their activities.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalBrain Research
Volume481
Issue number1
DOIs
Publication statusPublished - Feb 27 1989

Fingerprint

Growth Hormone-Releasing Hormone
Somatostatin
Hypothalamus
Electrons
Neurons
Arcuate Nucleus of Hypothalamus
Dendrites
Median Eminence
Synapses
Growth Hormone
Staining and Labeling
Light

Keywords

  • Arcuate nucleus
  • Electron microscopy
  • Growth hormone-releasing hormone
  • Hypothalamic periventricular nucleus
  • Immunocytochemistry
  • Somatostatin

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

@article{3e846ab0594e40a69b7b03cb1f27e455,
title = "Electron microscopic immunocytochemical evidence for the existence of bidirectional synaptic connections between growth hormone-releasing hormone- and somatostatin-containing neurons in the hypothalamus of the rat",
abstract = "Synaptic contacts between growth hormone-releasing hormone (GHRH)- and somatostatin-containing neurons were demonstrated in the rat hypothalamus by a double-staining immunocytochemical method at the electron microscopic level. Somatostatin-immunoreactive nerve terminals synapse on GHRH-positive dendrites and cell bodies in the arcuate nucleus. A fine network of GHRH-immunopositive nerve terminals was observed at the light microscopic level in the rostral part of the periventricular nucleus and in the dorsal part of the arcuate nucleus around somatostatin-containing neuronal elements. With the electron microscopic synaptic contact between GHRH-containing nerve terminals and somatostatin-containing dendrites are demonstrated. The reciprocal innervation between GHRH- and somatostatin-containing neurons that project to the median eminence and regulate growth hormone secretion must allow them to coordiante their activities.",
keywords = "Arcuate nucleus, Electron microscopy, Growth hormone-releasing hormone, Hypothalamic periventricular nucleus, Immunocytochemistry, Somatostatin",
author = "S{\'a}ndor Horv{\'a}th and M. Palk{\'o}vits and T. G{\"o}rcs and Akira Arimura",
year = "1989",
month = "2",
day = "27",
doi = "10.1016/0006-8993(89)90479-4",
language = "English",
volume = "481",
pages = "8--15",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Electron microscopic immunocytochemical evidence for the existence of bidirectional synaptic connections between growth hormone-releasing hormone- and somatostatin-containing neurons in the hypothalamus of the rat

AU - Horváth, Sándor

AU - Palkóvits, M.

AU - Görcs, T.

AU - Arimura, Akira

PY - 1989/2/27

Y1 - 1989/2/27

N2 - Synaptic contacts between growth hormone-releasing hormone (GHRH)- and somatostatin-containing neurons were demonstrated in the rat hypothalamus by a double-staining immunocytochemical method at the electron microscopic level. Somatostatin-immunoreactive nerve terminals synapse on GHRH-positive dendrites and cell bodies in the arcuate nucleus. A fine network of GHRH-immunopositive nerve terminals was observed at the light microscopic level in the rostral part of the periventricular nucleus and in the dorsal part of the arcuate nucleus around somatostatin-containing neuronal elements. With the electron microscopic synaptic contact between GHRH-containing nerve terminals and somatostatin-containing dendrites are demonstrated. The reciprocal innervation between GHRH- and somatostatin-containing neurons that project to the median eminence and regulate growth hormone secretion must allow them to coordiante their activities.

AB - Synaptic contacts between growth hormone-releasing hormone (GHRH)- and somatostatin-containing neurons were demonstrated in the rat hypothalamus by a double-staining immunocytochemical method at the electron microscopic level. Somatostatin-immunoreactive nerve terminals synapse on GHRH-positive dendrites and cell bodies in the arcuate nucleus. A fine network of GHRH-immunopositive nerve terminals was observed at the light microscopic level in the rostral part of the periventricular nucleus and in the dorsal part of the arcuate nucleus around somatostatin-containing neuronal elements. With the electron microscopic synaptic contact between GHRH-containing nerve terminals and somatostatin-containing dendrites are demonstrated. The reciprocal innervation between GHRH- and somatostatin-containing neurons that project to the median eminence and regulate growth hormone secretion must allow them to coordiante their activities.

KW - Arcuate nucleus

KW - Electron microscopy

KW - Growth hormone-releasing hormone

KW - Hypothalamic periventricular nucleus

KW - Immunocytochemistry

KW - Somatostatin

UR - http://www.scopus.com/inward/record.url?scp=0024556497&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024556497&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(89)90479-4

DO - 10.1016/0006-8993(89)90479-4

M3 - Article

C2 - 2565134

AN - SCOPUS:0024556497

VL - 481

SP - 8

EP - 15

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -