Electron microscope and high resoluation autoradiographic studies of the erythroblasts in haemoglobin H disease

S. N. Wickramasinghe, M. Hughes, S. Hollán, M. Horányi, J. Szelényi

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Abstract

Electron microscope studies of bone marrow from a patient with haemoglobin H (HbH) disease have revealed the presence of highly condensed branching intracytoplasmic inclusions within a proportion of the early and late polychromatic erythroblasts and reticulocytes. Intraerythroblastic inclusions of this type have not yet been described in any other haematological disorder and may well be a specific feature of HbH disease. Electron microscope autoradiographic studies of the marrow cells from the patient with HbH disease revealed that (1) active protein synthesis continues in inclusion-containing erythroblasts and (2) is a rapid precipitation of a considerable proportion of the newly synthesized free β chains onto the surfaces of preformed inclusions, presumably after conversion to HbH. These electron microscope and electron microscope autoradiographic findings are quite different from the findings previously reported in homozygous β thalassaemia using the same techniques.

Original languageEnglish
Pages (from-to)401-404
Number of pages4
JournalBritish Journal of Haematology
Volume45
Issue number3
Publication statusPublished - 1980

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alpha-Thalassemia
Erythroblasts
Electrons
Hemoglobin H
Bone Marrow
Thalassemia
Reticulocytes
Proteins

ASJC Scopus subject areas

  • Hematology

Cite this

Electron microscope and high resoluation autoradiographic studies of the erythroblasts in haemoglobin H disease. / Wickramasinghe, S. N.; Hughes, M.; Hollán, S.; Horányi, M.; Szelényi, J.

In: British Journal of Haematology, Vol. 45, No. 3, 1980, p. 401-404.

Research output: Contribution to journalArticle

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