Electron histochemistry of thiamine pyrophosphatase activity in the neuronal Golgi apparatus observed after axotomy and transneuronal deprivation

I. László, E. Knyihár-Csillik

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5 Citations (Scopus)

Abstract

Thiamine pyrophosphatase activity of the neuronal Golgi apparatus exhibits specific patterns, characterizing nerve cell types of the rat spinal cord at the light microscopal level. Electron histochemistry reveals TPPase activity within cisterns of the internal part of the dictiosomes and in vesicles associated with the Golgi system. According to electron microscopical studies performed on semi-thin (0.5 μ) sections, TPPase activity outlines a three-dimensional system of fenestrated cisterns and vesicles. In accord with literature data, axotomy of motoneurones results in a light microscopic decrease of dictiosomal TPPase activity and in an electron microscopic hypertrophy of the Golgi system. Electron histochemically, TPPase in the hypertrophied cisterns exhibits a sporadic, patchy localization, which is completely restored only in the state of restitution. On the contrary, transection of dorsal roots does not induce any light- or electron microscopic alterations in the TPPase activity of cells in the substantia gelatinosa Rolandi. Alterations of the Golgi system evoked by retrograde effects may serve to supply TPPase reserves in synaptic vesicles. Axotomy-induced alterations of neuronal TPPase reaction offer a methodological possibility for hodological studies.

Original languageEnglish
Pages (from-to)123-141
Number of pages19
JournalJournal of Neural Transmission
Volume36
Issue number2
DOIs
Publication statusPublished - Jun 1975

Fingerprint

Thiamine Pyrophosphatase
Axotomy
Golgi Apparatus
Electrons
Light
Substantia Gelatinosa
Spinal Nerve Roots
Synaptic Vesicles
Motor Neurons
Hypertrophy
Spinal Cord
Neurons

Keywords

  • axotomy
  • electron microscopy
  • Golgi apparatus
  • thiamine pyrophosphatase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

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abstract = "Thiamine pyrophosphatase activity of the neuronal Golgi apparatus exhibits specific patterns, characterizing nerve cell types of the rat spinal cord at the light microscopal level. Electron histochemistry reveals TPPase activity within cisterns of the internal part of the dictiosomes and in vesicles associated with the Golgi system. According to electron microscopical studies performed on semi-thin (0.5 μ) sections, TPPase activity outlines a three-dimensional system of fenestrated cisterns and vesicles. In accord with literature data, axotomy of motoneurones results in a light microscopic decrease of dictiosomal TPPase activity and in an electron microscopic hypertrophy of the Golgi system. Electron histochemically, TPPase in the hypertrophied cisterns exhibits a sporadic, patchy localization, which is completely restored only in the state of restitution. On the contrary, transection of dorsal roots does not induce any light- or electron microscopic alterations in the TPPase activity of cells in the substantia gelatinosa Rolandi. Alterations of the Golgi system evoked by retrograde effects may serve to supply TPPase reserves in synaptic vesicles. Axotomy-induced alterations of neuronal TPPase reaction offer a methodological possibility for hodological studies.",
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N2 - Thiamine pyrophosphatase activity of the neuronal Golgi apparatus exhibits specific patterns, characterizing nerve cell types of the rat spinal cord at the light microscopal level. Electron histochemistry reveals TPPase activity within cisterns of the internal part of the dictiosomes and in vesicles associated with the Golgi system. According to electron microscopical studies performed on semi-thin (0.5 μ) sections, TPPase activity outlines a three-dimensional system of fenestrated cisterns and vesicles. In accord with literature data, axotomy of motoneurones results in a light microscopic decrease of dictiosomal TPPase activity and in an electron microscopic hypertrophy of the Golgi system. Electron histochemically, TPPase in the hypertrophied cisterns exhibits a sporadic, patchy localization, which is completely restored only in the state of restitution. On the contrary, transection of dorsal roots does not induce any light- or electron microscopic alterations in the TPPase activity of cells in the substantia gelatinosa Rolandi. Alterations of the Golgi system evoked by retrograde effects may serve to supply TPPase reserves in synaptic vesicles. Axotomy-induced alterations of neuronal TPPase reaction offer a methodological possibility for hodological studies.

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