Electron beam treatment for tackling the escalating problems of antibiotic resistance: Eliminating the antimicrobial activity of wastewater matrices originating from erythromycin

László Szabó, Júlia Szabó, E. Illés, A. Kovács, Ágnes Belák, Csilla Mohácsi-Farkas, E. Takács, L. Wojnárovits

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10 Citations (Scopus)

Abstract

A synthetic effluent wastewater matrix was designed to be a kinetically appropriate reflection of a real sample while containing the antibiotic erythromycin at sufficiently high concentration for the microbiological assay. Following preliminary experiments on S. aureus isolates resistant and susceptible subtypes could be obtained against erythromycin. Agar diffusion and broth microdilution assays indicated antibacterial activity of the products forming in treated dilute aqueous solution of the antibiotic, however, also showed the applicability of EB treatment to eliminate their activity. In the complex wastewater matrix despite the high amount of secondary radicals forming from the matrix components (e.g. humic acid, HCO3) the elimination of the antibacterial activity is suggested to follow the stoichiometry of [rad]OH in respect to the antibiotic in line with dilute aqueous solution. Although there will be cases when secondary radicals will help degrading a molecule, the technology needs to be designed to the stoichiometric presence of [rad]OH according to the worst-case scenario approach.

Original languageEnglish
Pages (from-to)314-324
Number of pages11
JournalChemical Engineering Journal
Volume321
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

antimicrobial activity
antibiotic resistance
Antibiotics
Erythromycin
antibiotics
Electron beams
Wastewater
Anti-Bacterial Agents
wastewater
electron
matrix
Assays
aqueous solution
assay
Humic Substances
stoichiometry
Stoichiometry
agar
humic acid
Agar

Keywords

  • Advanced oxidation
  • Antimicrobial activity
  • Electron beam
  • Wastewater matrix
  • Wastewater treatment

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering

Cite this

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abstract = "A synthetic effluent wastewater matrix was designed to be a kinetically appropriate reflection of a real sample while containing the antibiotic erythromycin at sufficiently high concentration for the microbiological assay. Following preliminary experiments on S. aureus isolates resistant and susceptible subtypes could be obtained against erythromycin. Agar diffusion and broth microdilution assays indicated antibacterial activity of the products forming in treated dilute aqueous solution of the antibiotic, however, also showed the applicability of EB treatment to eliminate their activity. In the complex wastewater matrix despite the high amount of secondary radicals forming from the matrix components (e.g. humic acid, HCO3−) the elimination of the antibacterial activity is suggested to follow the stoichiometry of [rad]OH in respect to the antibiotic in line with dilute aqueous solution. Although there will be cases when secondary radicals will help degrading a molecule, the technology needs to be designed to the stoichiometric presence of [rad]OH according to the worst-case scenario approach.",
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T2 - Eliminating the antimicrobial activity of wastewater matrices originating from erythromycin

AU - Szabó, László

AU - Szabó, Júlia

AU - Illés, E.

AU - Kovács, A.

AU - Belák, Ágnes

AU - Mohácsi-Farkas, Csilla

AU - Takács, E.

AU - Wojnárovits, L.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - A synthetic effluent wastewater matrix was designed to be a kinetically appropriate reflection of a real sample while containing the antibiotic erythromycin at sufficiently high concentration for the microbiological assay. Following preliminary experiments on S. aureus isolates resistant and susceptible subtypes could be obtained against erythromycin. Agar diffusion and broth microdilution assays indicated antibacterial activity of the products forming in treated dilute aqueous solution of the antibiotic, however, also showed the applicability of EB treatment to eliminate their activity. In the complex wastewater matrix despite the high amount of secondary radicals forming from the matrix components (e.g. humic acid, HCO3−) the elimination of the antibacterial activity is suggested to follow the stoichiometry of [rad]OH in respect to the antibiotic in line with dilute aqueous solution. Although there will be cases when secondary radicals will help degrading a molecule, the technology needs to be designed to the stoichiometric presence of [rad]OH according to the worst-case scenario approach.

AB - A synthetic effluent wastewater matrix was designed to be a kinetically appropriate reflection of a real sample while containing the antibiotic erythromycin at sufficiently high concentration for the microbiological assay. Following preliminary experiments on S. aureus isolates resistant and susceptible subtypes could be obtained against erythromycin. Agar diffusion and broth microdilution assays indicated antibacterial activity of the products forming in treated dilute aqueous solution of the antibiotic, however, also showed the applicability of EB treatment to eliminate their activity. In the complex wastewater matrix despite the high amount of secondary radicals forming from the matrix components (e.g. humic acid, HCO3−) the elimination of the antibacterial activity is suggested to follow the stoichiometry of [rad]OH in respect to the antibiotic in line with dilute aqueous solution. Although there will be cases when secondary radicals will help degrading a molecule, the technology needs to be designed to the stoichiometric presence of [rad]OH according to the worst-case scenario approach.

KW - Advanced oxidation

KW - Antimicrobial activity

KW - Electron beam

KW - Wastewater matrix

KW - Wastewater treatment

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