Abstract
Electrical parameters were optimized for induction of the fusion of protoplasts formed from two auxotrophic (leu-, ade-) Mucor circinelloides strains. These proved to be a pulse voltage of 66 V, a pulse duration of 66 μs and six pulses at intervals of 1.0 s. Nutritionally complementing colonies were obtained at an average frequency up to 12.4%, which is substantially higher than achieved earlier with the PEG-Ca2+ method.
| Original language | English |
|---|---|
| Pages (from-to) | 607-610 |
| Number of pages | 4 |
| Journal | Biotechnology Techniques |
| Volume | 10 |
| Issue number | 8 |
| Publication status | Published - 1996 |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Biochemistry
Cite this
Electrofusion of Mucor circinelloides protoplasts. / Somogyvári, F.; Vágvölgyi, C.; Papp, T.; Ferenczy, L.
In: Biotechnology Techniques, Vol. 10, No. 8, 1996, p. 607-610.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Electrofusion of Mucor circinelloides protoplasts
AU - Somogyvári, F.
AU - Vágvölgyi, C.
AU - Papp, T.
AU - Ferenczy, L.
PY - 1996
Y1 - 1996
N2 - Electrical parameters were optimized for induction of the fusion of protoplasts formed from two auxotrophic (leu-, ade-) Mucor circinelloides strains. These proved to be a pulse voltage of 66 V, a pulse duration of 66 μs and six pulses at intervals of 1.0 s. Nutritionally complementing colonies were obtained at an average frequency up to 12.4%, which is substantially higher than achieved earlier with the PEG-Ca2+ method.
AB - Electrical parameters were optimized for induction of the fusion of protoplasts formed from two auxotrophic (leu-, ade-) Mucor circinelloides strains. These proved to be a pulse voltage of 66 V, a pulse duration of 66 μs and six pulses at intervals of 1.0 s. Nutritionally complementing colonies were obtained at an average frequency up to 12.4%, which is substantially higher than achieved earlier with the PEG-Ca2+ method.
UR - http://www.scopus.com/inward/record.url?scp=0029789252&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029789252&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0029789252
VL - 10
SP - 607
EP - 610
JO - Biotechnology Letters
JF - Biotechnology Letters
SN - 0141-5492
IS - 8
ER -