Electrofusion of Mucor circinelloides protoplasts

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Electrical parameters were optimized for induction of the fusion of protoplasts formed from two auxotrophic (leu-, ade-) Mucor circinelloides strains. These proved to be a pulse voltage of 66 V, a pulse duration of 66 μs and six pulses at intervals of 1.0 s. Nutritionally complementing colonies were obtained at an average frequency up to 12.4%, which is substantially higher than achieved earlier with the PEG-Ca2+ method.

Original languageEnglish
Pages (from-to)607-610
Number of pages4
JournalBiotechnology Techniques
Volume10
Issue number8
Publication statusPublished - 1996

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Mucor
Protoplasts
Polyethylene glycols
Fusion reactions
Electric potential

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biochemistry

Cite this

Electrofusion of Mucor circinelloides protoplasts. / Somogyvári, F.; Vágvölgyi, C.; Papp, T.; Ferenczy, L.

In: Biotechnology Techniques, Vol. 10, No. 8, 1996, p. 607-610.

Research output: Contribution to journalArticle

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