Electrofusion of Mucor circinelloides protoplasts

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Electrical parameters were optimized for induction of the fusion of protoplasts formed from two auxotrophic (leu-, ade-) Mucor circinelloides strains. These proved to be a pulse voltage of 66 V, a pulse duration of 66 μs and six pulses at intervals of 1.0 s. Nutritionally complementing colonies were obtained at an average frequency up to 12.4%, which is substantially higher than achieved earlier with the PEG-Ca2+ method.

Original languageEnglish
Pages (from-to)607-610
Number of pages4
JournalBiotechnology Techniques
Issue number8
Publication statusPublished - Jan 1 1996


ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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