Electrochemical calibration of in vivo brain dialysis samplers

G. Juhász, J. Tarcali, K. Pungor, E. Pungor

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Measurement of state-related changes in the concentration of an endogenous substance using the in vivo dialysis technique requires a definition of the factors which relate the concentration detected in the outflowing perfusate to the concentration actually occurring in the extracellular space in which the sampler is located. In determine these factors, a rapid and highly accurate detector system is required which measures the concentration recovery in the perfusate and the dead space of the entire sampling system. To reduce the limitations of the microdialysis technique, two electrochemical microcells were developed for calibration of dialysis probes by computerized voltammetry. The electrochemically calibrated samplers were implanted into freely moving cats to measure concentration changes of monoamine metabolites in synchrony with sleep stages identified by polygraphy in order to demonstrate the applicability of electrochemical calibration in dialysis methods used in behavioral investigations.

Original languageEnglish
Pages (from-to)131-137
Number of pages7
JournalJournal of Neuroscience Methods
Volume29
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Calibration
Dialysis
Brain
Electrochemical Techniques
Sleep Stages
Microdialysis
Extracellular Space
Cats

Keywords

  • Brain dialysis
  • Calibration electrochemistry
  • In vivo

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Electrochemical calibration of in vivo brain dialysis samplers. / Juhász, G.; Tarcali, J.; Pungor, K.; Pungor, E.

In: Journal of Neuroscience Methods, Vol. 29, No. 2, 1989, p. 131-137.

Research output: Contribution to journalArticle

Juhász, G. ; Tarcali, J. ; Pungor, K. ; Pungor, E. / Electrochemical calibration of in vivo brain dialysis samplers. In: Journal of Neuroscience Methods. 1989 ; Vol. 29, No. 2. pp. 131-137.
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