Electrochemical and Photometric Detection of Plasmin by Specific Peptide Substrate

Gabriela Castillo, Kinga Pribransky, G. Mező, László Kocsis, A. Csámpai, Krisztina Németh, Z. Keresztes, Tibor Hianik

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We report a comparative analysis of plasmin detection by means of electrochemical biosensor and photometric assays. The biosensor has been based on ferrocene-modified peptide (Fc-P) specific to plasmin. Fc-P substrate is enzymatically cleaved by plasmin and the short fragment of peptide contained Fc is released causing decrease of redox current measured by cyclic voltammetry. The biosensor was tested in the range 1-20nM of freshly prepared plasmin and it was highly selective (LOD=0.56±0.03nM) demonstrating lower reaction to other proteins such as thrombin and β-casein. The concentration of plasmin has been determined also by a photometric analysis based on UV-vis spectroscopy (LOD=3.68±0.04nM). The assay has been validated in milk samples.

Original languageEnglish
Pages (from-to)789-798
Number of pages10
JournalElectroanalysis
Volume27
Issue number3
DOIs
Publication statusPublished - Mar 1 2015

Fingerprint

Fibrinolysin
Biosensors
Peptides
Assays
Substrates
Casein
Ultraviolet spectroscopy
Cyclic voltammetry
Peptide Fragments
Proteins
Caseins
Thrombin
ferrocene

Keywords

  • Electrochemical detection
  • Ferrocene
  • Peptide substrate
  • Plasmin
  • UV-vis spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Electrochemical and Photometric Detection of Plasmin by Specific Peptide Substrate. / Castillo, Gabriela; Pribransky, Kinga; Mező, G.; Kocsis, László; Csámpai, A.; Németh, Krisztina; Keresztes, Z.; Hianik, Tibor.

In: Electroanalysis, Vol. 27, No. 3, 01.03.2015, p. 789-798.

Research output: Contribution to journalArticle

Castillo, Gabriela ; Pribransky, Kinga ; Mező, G. ; Kocsis, László ; Csámpai, A. ; Németh, Krisztina ; Keresztes, Z. ; Hianik, Tibor. / Electrochemical and Photometric Detection of Plasmin by Specific Peptide Substrate. In: Electroanalysis. 2015 ; Vol. 27, No. 3. pp. 789-798.
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