Electro-fused isolated wheat (Triticum aestivum L.) gametes develop into multicellular structures

Miklós Kovács, B. Barnabás, Erhard Kranz

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The electrofusion-mediated fertilization of single egg cells of wheat with isolated individually selected wheat sperm cells was successfully carried out for the first time. On average the fusion frequency was 30% but under optimal conditions it was possible to reach as much as 55%. Two days after electric fusion 60% of the fusion products started to divide, 88.5% of them forming multicellular structures and in a few cases microcalluses. The culture of single unfertilized egg cells with or without the application of AC field and electric pulses induced no cell division. The egg cells and fusion products were cultured in a maize feeder-cell system.

Original languageEnglish
Pages (from-to)178-180
Number of pages3
JournalPlant Cell Reports
Volume15
Issue number3-4
DOIs
Publication statusPublished - Dec 1995

Fingerprint

ova
germ cells
Triticum aestivum
wheat
electrofusion
cell division
spermatozoa
cells
corn

ASJC Scopus subject areas

  • Plant Science

Cite this

Electro-fused isolated wheat (Triticum aestivum L.) gametes develop into multicellular structures. / Kovács, Miklós; Barnabás, B.; Kranz, Erhard.

In: Plant Cell Reports, Vol. 15, No. 3-4, 12.1995, p. 178-180.

Research output: Contribution to journalArticle

@article{38b1b72aea0049a886ed625be3a54a2d,
title = "Electro-fused isolated wheat (Triticum aestivum L.) gametes develop into multicellular structures",
abstract = "The electrofusion-mediated fertilization of single egg cells of wheat with isolated individually selected wheat sperm cells was successfully carried out for the first time. On average the fusion frequency was 30{\%} but under optimal conditions it was possible to reach as much as 55{\%}. Two days after electric fusion 60{\%} of the fusion products started to divide, 88.5{\%} of them forming multicellular structures and in a few cases microcalluses. The culture of single unfertilized egg cells with or without the application of AC field and electric pulses induced no cell division. The egg cells and fusion products were cultured in a maize feeder-cell system.",
author = "Mikl{\'o}s Kov{\'a}cs and B. Barnab{\'a}s and Erhard Kranz",
year = "1995",
month = "12",
doi = "10.1007/BF00193715",
language = "English",
volume = "15",
pages = "178--180",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "3-4",

}

TY - JOUR

T1 - Electro-fused isolated wheat (Triticum aestivum L.) gametes develop into multicellular structures

AU - Kovács, Miklós

AU - Barnabás, B.

AU - Kranz, Erhard

PY - 1995/12

Y1 - 1995/12

N2 - The electrofusion-mediated fertilization of single egg cells of wheat with isolated individually selected wheat sperm cells was successfully carried out for the first time. On average the fusion frequency was 30% but under optimal conditions it was possible to reach as much as 55%. Two days after electric fusion 60% of the fusion products started to divide, 88.5% of them forming multicellular structures and in a few cases microcalluses. The culture of single unfertilized egg cells with or without the application of AC field and electric pulses induced no cell division. The egg cells and fusion products were cultured in a maize feeder-cell system.

AB - The electrofusion-mediated fertilization of single egg cells of wheat with isolated individually selected wheat sperm cells was successfully carried out for the first time. On average the fusion frequency was 30% but under optimal conditions it was possible to reach as much as 55%. Two days after electric fusion 60% of the fusion products started to divide, 88.5% of them forming multicellular structures and in a few cases microcalluses. The culture of single unfertilized egg cells with or without the application of AC field and electric pulses induced no cell division. The egg cells and fusion products were cultured in a maize feeder-cell system.

UR - http://www.scopus.com/inward/record.url?scp=0029197417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029197417&partnerID=8YFLogxK

U2 - 10.1007/BF00193715

DO - 10.1007/BF00193715

M3 - Article

VL - 15

SP - 178

EP - 180

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 3-4

ER -