Elastic properties of protein gels obtained by three-phase partitioning

R. Borbás, E. Kiss, M. Nagy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A special combination of alcoholic precipitation and salting out of proteins was described as three-phase partitioning [1]. Proteins are accumulated in a coherent middle phase in the partitioning system composed of tert-butanol, ammonium sulphate and water. The mechanical properties of the middle phase were determined in model systems using bovine serum albumin (BSA) or ovalbumin. The stress-deformation relationship obtained by uniaxial compression showed the elastic behaviour of the middle phase. It was found that the elastic modulus of the protein gel changed with stress. The middle phase became harder upon compression, possibly owing to some change in structure. The viscoelastic properties were determined by measuring the deformation as a function of time. The BSA gels showed higher elastic and viscous deformation and lower viscosity than the ovalbumin gels. The rheological behaviour of protein gels is consistent with the previous results obtained from the analysis of middle-phase components, which indicate that the middle phase formed in three-phase partitioning is an emulsion gel.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalProgress in Colloid and Polymer Science
Volume117
Publication statusPublished - 2001

Fingerprint

Gels
elastic properties
gels
proteins
Proteins
Ovalbumin
Bovine Serum Albumin
albumins
serums
tert-Butyl Alcohol
ammonium sulfates
elastic deformation
Ammonium Sulfate
Emulsions
Butenes
emulsions
modulus of elasticity
Elastic moduli
mechanical properties
Viscosity

Keywords

  • Emulsion gel
  • Protein separation
  • Rheology of bovine serum albumin and ovalbumin gels
  • Three-phase partitioning

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Chemistry (miscellaneous)
  • Colloid and Surface Chemistry
  • Polymers and Plastics

Cite this

Elastic properties of protein gels obtained by three-phase partitioning. / Borbás, R.; Kiss, E.; Nagy, M.

In: Progress in Colloid and Polymer Science, Vol. 117, 2001, p. 189-194.

Research output: Contribution to journalArticle

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