Elastic fiber formation

A dynamic view of extracellular matrix assembly using timer reporters

Beth A. Kozel, Brenda J. Rongish, A. Czirók, Julia Zach, Charles D. Little, Elaine C. Davis, Russell H. Knutsen, Jessica E. Wagenseil, Marilyn A. Levy, Robert P. Mecham

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

To study the dynamics of elastic fiber assembly, mammalian cells were transfected with a cDNA construct encoding bovine tropoelastin in frame with the Timer reporter. Timer is a derivative of the DsRed fluorescent protein that changes from green to red overtime and, hence, can be used to distinguish new from old elastin. Using dynamic imaging microscopy, we found that the first step in elastic fiber formation is the appearance of small cell surface-associated elastin globules that increased in size with time (microassembly). The elastin globules are eventually transferred to pre-existing elastic fibers in the extracellular matrix where they coalesce into larger structures (macroassembly). Mechanical forces associated with cell movement help shape the forming, extracellular elastic fiber network. Time-lapse imaging combined with the use of Timer constructs provides unique tools for studying the temporal and spatial aspects of extracellular matrix formation by live cells.

Original languageEnglish
Pages (from-to)87-96
Number of pages10
JournalJournal of Cellular Physiology
Volume207
Issue number1
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Elastic Tissue
Extracellular Matrix
Elastin
Fibers
Tropoelastin
Cells
Time-Lapse Imaging
Imaging techniques
Green Fluorescent Proteins
Cell Movement
Microscopy
Microscopic examination
Complementary DNA
Derivatives

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Kozel, B. A., Rongish, B. J., Czirók, A., Zach, J., Little, C. D., Davis, E. C., ... Mecham, R. P. (2006). Elastic fiber formation: A dynamic view of extracellular matrix assembly using timer reporters. Journal of Cellular Physiology, 207(1), 87-96. https://doi.org/10.1002/jcp.20546

Elastic fiber formation : A dynamic view of extracellular matrix assembly using timer reporters. / Kozel, Beth A.; Rongish, Brenda J.; Czirók, A.; Zach, Julia; Little, Charles D.; Davis, Elaine C.; Knutsen, Russell H.; Wagenseil, Jessica E.; Levy, Marilyn A.; Mecham, Robert P.

In: Journal of Cellular Physiology, Vol. 207, No. 1, 04.2006, p. 87-96.

Research output: Contribution to journalArticle

Kozel, BA, Rongish, BJ, Czirók, A, Zach, J, Little, CD, Davis, EC, Knutsen, RH, Wagenseil, JE, Levy, MA & Mecham, RP 2006, 'Elastic fiber formation: A dynamic view of extracellular matrix assembly using timer reporters', Journal of Cellular Physiology, vol. 207, no. 1, pp. 87-96. https://doi.org/10.1002/jcp.20546
Kozel, Beth A. ; Rongish, Brenda J. ; Czirók, A. ; Zach, Julia ; Little, Charles D. ; Davis, Elaine C. ; Knutsen, Russell H. ; Wagenseil, Jessica E. ; Levy, Marilyn A. ; Mecham, Robert P. / Elastic fiber formation : A dynamic view of extracellular matrix assembly using timer reporters. In: Journal of Cellular Physiology. 2006 ; Vol. 207, No. 1. pp. 87-96.
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