Between 1992 and 2000, a total of 4,173 rotavirus-positive samples were collected from two areas of Hungary. Of these, 2,020 specimens (48.4%) were analyzed for G serotype, using monoclonal antibody-based immunoassay and reverse transcription-PCR. By the two methods, 1,789 samples were specified as G1 (62%), G2 (12.2%), G3 (1.4%), G4 (6.4%), G6 (1.0%), G9 (2.9%), or mixed infection (2.6%), and the remaining 231 (11.4%) could not be G typed. The linkage between G and P type, subgroup specificity, and RNA profile was investigated with a sample subset. Among these specimens, we identified both the four globally common strains (P, G1 subgroup II (sgII); P,G2 sgI; P,G3 sgII; and P,G4 sgII) and six uncommon strains (P,G4 sgII; P,G3 sgI; P,G6 sgI; P,G6 sgI; P,G9 sgII; and P,G9 sgI). All strains with P, P, P, and P specificities had a long electropherotype, whereas most of those carrying a P specificity were associated with a short electropherotype. Although once considered to be rare, P,G6 and P,G9 rotavirus strains represent potentially important new serotypes in Hungary.
ASJC Scopus subject areas
- Microbiology (medical)