Efficient transposon mutagenesis in a wide range of phytopathogenic gram-negative bacteria

A. Czelleng, Z. Bozsó, P. Ott, E. Besenyei, G. J. Varga, A. Szatmári, E. Szabó, L. R. Zsiros, Z. Klement

Research output: Contribution to journalArticle

Abstract

Compared to the known method of conjugation the frequency of transposon mutagenesis following conjugation was enhanced 11-fold by two hours of pre-incubation of recipient Pseudomonas viridiflava 1 on conjugation media. The increased frequency was f = 1.3 x 10-4. In other species of Pseudomonas, Pectobacteira and Xanthomonas high rates of transposon mutants were similarly obtained; however, in these strains the increased frequency was less than 5-fold.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalActa Phytopathologica et Entomologica Hungarica
Volume44
Issue number1
DOIs
Publication statusPublished - Jun 1 2009

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plant pathogenic bacteria
Gram-negative bacteria
mutagenesis
transposons
Pseudomonas viridiflava
Xanthomonas
Pseudomonas
mutants
methodology

Keywords

  • Inter specific conjugation
  • Pre-incubation
  • Transposon mutagenesis

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

Efficient transposon mutagenesis in a wide range of phytopathogenic gram-negative bacteria. / Czelleng, A.; Bozsó, Z.; Ott, P.; Besenyei, E.; Varga, G. J.; Szatmári, A.; Szabó, E.; Zsiros, L. R.; Klement, Z.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 44, No. 1, 01.06.2009, p. 19-24.

Research output: Contribution to journalArticle

Czelleng, A, Bozsó, Z, Ott, P, Besenyei, E, Varga, GJ, Szatmári, A, Szabó, E, Zsiros, LR & Klement, Z 2009, 'Efficient transposon mutagenesis in a wide range of phytopathogenic gram-negative bacteria', Acta Phytopathologica et Entomologica Hungarica, vol. 44, no. 1, pp. 19-24. https://doi.org/10.1556/APhyt.44.2009.1.3
Czelleng, A. ; Bozsó, Z. ; Ott, P. ; Besenyei, E. ; Varga, G. J. ; Szatmári, A. ; Szabó, E. ; Zsiros, L. R. ; Klement, Z. / Efficient transposon mutagenesis in a wide range of phytopathogenic gram-negative bacteria. In: Acta Phytopathologica et Entomologica Hungarica. 2009 ; Vol. 44, No. 1. pp. 19-24.
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