Efficient light harvesting in a dark, hot, acidic environment: The structure and function of PSI-LHCI from Galdieria sulphuraria

Balakumar Thangaraj, Craig C. Jolley, Iosifina Sarrou, Jelle B. Bultema, Jason Greyslak, Julian P. Whitelegge, Su Lin, Roman Kouřil, Rajagopal Subramanyam, Egbert J. Boekema, Petra Fromme

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22 Citations (Scopus)

Abstract

Photosystem I-light harvesting complex I (PSI-LHCI) was isolated from the thermoacidophilic red alga Galdieria sulphuraria, and its structure, composition, and light-harvesting function were characterized by electron microscopy, mass spectrometry, and ultrafast optical spectroscopy. The results show that Galdieria PSI is a monomer with core features similar to those of PSI from green algae, but with significant differences in shape and size. A comparison with the crystal structure of higher plant (pea) PSI-LHCI indicates that Galdieria PSI binds seven to nine light-harvesting proteins. Results from ultrafast optical spectroscopy show that the functional coupling of the LHCI proteins to the PSI core is tighter than in other eukaryotic PSI-LHCI systems reported thus far. This tight coupling helps Galdieria perform efficient light harvesting under the low-light conditions present in its natural endolithic habitat.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalBiophysical journal
Volume100
Issue number1
DOIs
Publication statusPublished - Jan 5 2011

ASJC Scopus subject areas

  • Biophysics

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    Thangaraj, B., Jolley, C. C., Sarrou, I., Bultema, J. B., Greyslak, J., Whitelegge, J. P., Lin, S., Kouřil, R., Subramanyam, R., Boekema, E. J., & Fromme, P. (2011). Efficient light harvesting in a dark, hot, acidic environment: The structure and function of PSI-LHCI from Galdieria sulphuraria. Biophysical journal, 100(1), 135-143. https://doi.org/10.1016/j.bpj.2010.09.069