Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes

Li Ying Sung, Ching Chien Chang, Tomokazu Amano, Chih Jen Lin, Misa Amano, Stephen B. Treaster, Jie Xu, Wei Fang Chang, P. Nagy, Xiangzhong Yang, X. Cindy Tian

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Deriving histocompatible embryonic stem (ES) cells by somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA) requires fresh oocytes, which prevents their applications in humans. Here, we evaluated the efficiency of deriving ES cells from mature metaphase II (MII) and immature metaphase I (MI) vitrified oocytes, by PA or SCNT, in a mouse model. We successfully generated ES cell lines from PA (MII and MI) and SCNT (MII and MI) blastocysts. These cell lines expressed genes and antigens characteristic of pluripotent ES cells and produced full-term pups upon tetraploid embryo complementation. This study established an animal model for efficient generation of patient-specific ES cell lines using cryopreserved oocytes. This is a major step forward in the application of therapeutic cloning and parthenogenetic technology in human regenerative medicine and will serve as an important alternative to the iPS cell technology in countries/regions where these technologies are permitted.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalCellular Reprogramming
Volume12
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

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Embryo Transfer
Metaphase
Embryonic Stem Cells
Oocytes
Technology
Cell Line
Pluripotent Stem Cells
Regenerative Medicine
Tetraploidy
Blastocyst
Organism Cloning
Embryonic Structures
Animal Models
Antigens
Genes

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Cell Biology

Cite this

Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes. / Sung, Li Ying; Chang, Ching Chien; Amano, Tomokazu; Lin, Chih Jen; Amano, Misa; Treaster, Stephen B.; Xu, Jie; Chang, Wei Fang; Nagy, P.; Yang, Xiangzhong; Tian, X. Cindy.

In: Cellular Reprogramming, Vol. 12, No. 2, 01.04.2010, p. 203-211.

Research output: Contribution to journalArticle

Sung, LY, Chang, CC, Amano, T, Lin, CJ, Amano, M, Treaster, SB, Xu, J, Chang, WF, Nagy, P, Yang, X & Tian, XC 2010, 'Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes', Cellular Reprogramming, vol. 12, no. 2, pp. 203-211. https://doi.org/10.1089/cell.2009.0072
Sung, Li Ying ; Chang, Ching Chien ; Amano, Tomokazu ; Lin, Chih Jen ; Amano, Misa ; Treaster, Stephen B. ; Xu, Jie ; Chang, Wei Fang ; Nagy, P. ; Yang, Xiangzhong ; Tian, X. Cindy. / Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes. In: Cellular Reprogramming. 2010 ; Vol. 12, No. 2. pp. 203-211.
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