Efficiency of different marker systems for genotype fingerprinting and for genetic diversity studies in barley (Hordeum vulgare L.)

Klara Meszaros, Ildiko Karsai, Csaba Kuti, Judit Banyai, Laszlo Lang, Zoltan Bedo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Genetic relationships between 38 barley genotypes were determined with the aid of 36 RAPD, 54 STS and 26 SSR markers. The dendrogram groups showed high coincidence with growth habit and ear type. There were significant correlations between the Jaccard coefficients obtained using the matrices of each single marker type and their combined matrix. When the varieties were grouped using markers with above-average Polymorphic Information Content (PIC) values, the same groups were obtained as when using all markers, outlining their usefulness for estimating diversity between the varieties. Three RAPD or four SSR primers were sufficient to distinguish all the barley varieties from each other. The applicability of the various types of primers differed. The STS markers could best be used for estimating relationships between the varieties and the SSR markers for distinguishing genotypes from each other, while RAPD markers could be employed both for estimating the relationships between varieties and for variety identification.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalSouth African Journal of Botany
Volume73
Issue number1
DOIs
Publication statusPublished - Jan 1 2007

Keywords

  • Barley
  • Diversity
  • Genotype fingerprinting
  • Molecular markers

ASJC Scopus subject areas

  • Plant Science

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