Ucinok Zeel comp. na experimentalnu osteoartrozu v kolene kralika

Translated title of the contribution: Effects of Zeel comp. on experimental osteoarthritis in rabbit knee

M. Stancikova, M. Bely, K. Svik, H. W. Metelmann, M. W. Schmolz, R. Istok, R. Fano

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Objective: Zeel comp. is a mixture of homeopathic extracts used in antihomotoxic medicine of osteoarthrosis. The clinical study in patients with gonarthrosis showed a pronounced beneficial effect of this compound. The aim of study: The evaluation of the Zeel comp. effect on the articular cartilage of knee joint during development of osteoarthrosis in rabbits following anterior cruciate ligament transsection (ACLT). Methods: A total of 12 New Zealand White (NZW) male rabbits were used in this study. The rabbits were randomly divided into two groups of 6 animals after surgical ACLT of right knees and simple arthrotomy of their contra-lateral left knees: osteoarthritic untreated group (OA) and Zeel comp. treated group. OA group received intra-articular injection of sterile saline and the treated group injected in the same manner with Zeel comp. into the right ACLT knee. At the same time both groups received intraarticular injection of sterile saline into the left knees (Sham controls). Zeel comp. or saline were injected intraarticularly twice a week, immediately after surgery until sacrifice at 9 weeks. The parameters tested were gross morphology, histology as well as urinary pyridinoline (Pyr). Most important results: Morphological changes in osteoarthritic group without treatment were seen on both, medial and lateral region, but markedly on medial condyle of the ACLT right knee. The articular cartilage was characterised by a rough and hypertrophic appearance with severe erosions. The severity of cartilage damage was generally lower in Zeel comp. group in comparison with OA group. The gross morphological examination of contralateral sham controls revealed very little or no changes. Gross morphological grading of cartilage damage showed significantly lower extent of damage in Zeel comp. group. Histological evidence for cartilage degeneration was observed in the ACLT knees of both treated and untreated rabbits. In OA controls the articular cartilage showed degenerative changes, including: rough surface, loss of superficial layer, erosion, fissures, irregular arrangement and form of chondrocytes. In the osteoarthritic group treated with Zeel comp. the signs of cartilage degeneration of femoral condyles were limited. The mean global histopathological score in the Zeel comp. group was significantly lower in medial condyles (20.70±0.64, p<0.05) in comparison with saline treated OA group (23.40±0.54). Similar results were observed in lateral condyles. Biochemical determinations showed significantly higher concentrations of urinary Pyr in the OA group compared to healthy rabbits during the whole period of the study (9 weeks), indicating a higher collagen degradation of cartilage and subchondral bone in the osteoarthritic animals. The urinary levels of rabbits treated with Zeel comp. was clearly lower compared to the OA controls. Conclusion: The results of this study showed that, intraarticular Zeel comp. injections did not completely prevent the development of joint damage in the ACLT rabbit model of osteoarthrosis, however the results of our morphological and histological experiments as well as the biochemical findings demonstrate a significant and substantial decrease in severity of the damage caused in condyle cartilage and in chondrocytes.

Translated title of the contributionEffects of Zeel comp. on experimental osteoarthritis in rabbit knee
Original languageSlovak
Pages (from-to)101-108
Number of pages8
Issue number3
Publication statusPublished - Jan 1 1999


  • Gross morphology
  • Histopathology
  • Osteoarthrosis
  • Pyridinoline
  • Rabbit
  • Zeel comp

ASJC Scopus subject areas

  • Rheumatology

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  • Cite this

    Stancikova, M., Bely, M., Svik, K., Metelmann, H. W., Schmolz, M. W., Istok, R., & Fano, R. (1999). Ucinok Zeel comp. na experimentalnu osteoartrozu v kolene kralika. Rheumatologia, 13(3), 101-108.