Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes

Duangjai Boonkusol, Tassanee Faisaikarm, A. Dinnyés, Yindee Kitiyanant

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The purpose of the present study was to investigate the effects of two vitrification procedures on developmental capacity and ultrastructural changes of matured swamp buffalo oocytes. In vitro-matured oocytes were vitrified by using 35 and 40% ethylene glycol as vitrification solution for solid surface vitrification (SSV) and in-straw vitrification (ISV), respectively. Survival rate of vitrified-warmed oocytes, evaluated on the basis of ooplasm homogeneity, oolemma integrity and zona pellucida intactness, as well as parthenogenetic blastocyst rates of vitrified-warmed oocytes were significantly higher with SSV (89.3 and 13.6%, respectively) than ISV (81.8 and 5.5%, respectively). However, they were still significantly lower than that of control oocytes (100 and 34.2%, respectively). For examining the ultrastructural changes, fresh, VS-exposed (ISV and SSV), and vitrified-warmed (ISV and SSV) oocytes were processed for transmission electron microscopy. In VS-exposed oocytes, reduction of microvilli abundance and damage of mitochondrial membrane were found only in the ISV group. In vitrified-warmed oocytes, however, it was clear that both methods of vitrification induced profound ultrastructural modifications to microvilli, mitochondria, oolemma and cortical granules as well as to the size and position of vesicles. Damaged mitochondria were, however, more abundant in ISV vitrified oocytes than in SSV vitrified oocytes, which correlated with the developmental data, showing the superiority of the SSV method. The present study demonstrated the feasibility of vitrification of in vitro-matured swamp buffalo oocytes.

Original languageEnglish
Pages (from-to)383-391
Number of pages9
JournalReproduction, Fertility and Development
Volume19
Issue number2
DOIs
Publication statusPublished - 2007

Fingerprint

Vitrification
Wetlands
Buffaloes
vitrification
Oocytes
buffaloes
ultrastructure
oocytes
straw
In Vitro Techniques
Microvilli
microvilli
Mitochondria
mitochondria
cortical granules
Zona Pellucida
zona pellucida
Ethylene Glycol
ethylene glycol
Mitochondrial Membranes

Keywords

  • Cryopreservation
  • Matured oocyte

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology

Cite this

Effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (Bubalus bubalis) oocytes. / Boonkusol, Duangjai; Faisaikarm, Tassanee; Dinnyés, A.; Kitiyanant, Yindee.

In: Reproduction, Fertility and Development, Vol. 19, No. 2, 2007, p. 383-391.

Research output: Contribution to journalArticle

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