Effects of vitamin D3 derivative -calcitriol on pharmacological reactivity of aortic rings in a rodent PCOS model

Gabriella Masszi, Agnes Novak, Robert Tarszabo, Eszter Maria Horvath, Anna Buday, Eva Ruisanchez, A. Tőkés, Levente Sara, Rita Benko, G. Nádasy, Csaba Revesz, P. Hamar, Zoltán Benyó, S. Várbíró

Research output: Contribution to journalArticle

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Abstract

Background: The aim of this study was to examine the effects of the hyperandrogenic state in dihydrotestosterone (DHT)-induced polycystic ovary syndrome (PCOS), the vascular responses to different vasoactive agents, and the modulatory role of vitamin D3. Methods: APCOS model was induced by DHT application in 20 femaleWistar rats. Ten of the DHT treated rats simultaneously received calcitriol treatment. After 10 weeks, myographs were used to test the reactivity of isolated thoracic aortic rings to norepinephrine and acetylcholine. Thereafter, the vascular rings were incubated with the NO-synthase blocker (nitro-L-Arginine methyl ester) or the cyclooxygenase inhibitor (indomethacin) for 20 min, and the effects of norepinephrine and acetylcholine were re-evaluated. Results: Norepinephrine-induced vasoconstriction was enhanced after DHT treatment, but this effect was attenuated by calcitriol administration. Vasorelaxation of DHT-treated thoracic aortic rings was impaired, but this could be partly reversed by calcitriol application. Impaired NO-dependent vasorelaxation in DHT-treated animals was mostly reversed by concomitant calcitriol administration, but this effect was diminished by prostanoid-dependent vasoconstriction. Conclusions: These studies show that the enhanced sensitivity to vasoconstrictors and impaired NO-dependent vasorelaxation in hyperandrogenic PCOS rats could be partially reversed by calcitriol treatment.

Original languageEnglish
Pages (from-to)476-483
Number of pages8
JournalPharmacological Reports
Volume65
Issue number2
Publication statusPublished - 2013

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Dihydrotestosterone
Calcitriol
Cholecalciferol
Polycystic Ovary Syndrome
Rodentia
Pharmacology
Vasodilation
Norepinephrine
Vasoconstriction
Acetylcholine
Blood Vessels
Thorax
Cyclooxygenase Inhibitors
Vasoconstrictor Agents
Nitric Oxide Synthase
Indomethacin
Prostaglandins

Keywords

  • Aorta
  • Calcitriol
  • DHT
  • NO
  • PCOS
  • Vascular reactivity
  • Vitamin D3

ASJC Scopus subject areas

  • Pharmacology

Cite this

Masszi, G., Novak, A., Tarszabo, R., Horvath, E. M., Buday, A., Ruisanchez, E., ... Várbíró, S. (2013). Effects of vitamin D3 derivative -calcitriol on pharmacological reactivity of aortic rings in a rodent PCOS model. Pharmacological Reports, 65(2), 476-483.

Effects of vitamin D3 derivative -calcitriol on pharmacological reactivity of aortic rings in a rodent PCOS model. / Masszi, Gabriella; Novak, Agnes; Tarszabo, Robert; Horvath, Eszter Maria; Buday, Anna; Ruisanchez, Eva; Tőkés, A.; Sara, Levente; Benko, Rita; Nádasy, G.; Revesz, Csaba; Hamar, P.; Benyó, Zoltán; Várbíró, S.

In: Pharmacological Reports, Vol. 65, No. 2, 2013, p. 476-483.

Research output: Contribution to journalArticle

Masszi, G, Novak, A, Tarszabo, R, Horvath, EM, Buday, A, Ruisanchez, E, Tőkés, A, Sara, L, Benko, R, Nádasy, G, Revesz, C, Hamar, P, Benyó, Z & Várbíró, S 2013, 'Effects of vitamin D3 derivative -calcitriol on pharmacological reactivity of aortic rings in a rodent PCOS model', Pharmacological Reports, vol. 65, no. 2, pp. 476-483.
Masszi G, Novak A, Tarszabo R, Horvath EM, Buday A, Ruisanchez E et al. Effects of vitamin D3 derivative -calcitriol on pharmacological reactivity of aortic rings in a rodent PCOS model. Pharmacological Reports. 2013;65(2):476-483.
Masszi, Gabriella ; Novak, Agnes ; Tarszabo, Robert ; Horvath, Eszter Maria ; Buday, Anna ; Ruisanchez, Eva ; Tőkés, A. ; Sara, Levente ; Benko, Rita ; Nádasy, G. ; Revesz, Csaba ; Hamar, P. ; Benyó, Zoltán ; Várbíró, S. / Effects of vitamin D3 derivative -calcitriol on pharmacological reactivity of aortic rings in a rodent PCOS model. In: Pharmacological Reports. 2013 ; Vol. 65, No. 2. pp. 476-483.
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AU - Novak, Agnes

AU - Tarszabo, Robert

AU - Horvath, Eszter Maria

AU - Buday, Anna

AU - Ruisanchez, Eva

AU - Tőkés, A.

AU - Sara, Levente

AU - Benko, Rita

AU - Nádasy, G.

AU - Revesz, Csaba

AU - Hamar, P.

AU - Benyó, Zoltán

AU - Várbíró, S.

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N2 - Background: The aim of this study was to examine the effects of the hyperandrogenic state in dihydrotestosterone (DHT)-induced polycystic ovary syndrome (PCOS), the vascular responses to different vasoactive agents, and the modulatory role of vitamin D3. Methods: APCOS model was induced by DHT application in 20 femaleWistar rats. Ten of the DHT treated rats simultaneously received calcitriol treatment. After 10 weeks, myographs were used to test the reactivity of isolated thoracic aortic rings to norepinephrine and acetylcholine. Thereafter, the vascular rings were incubated with the NO-synthase blocker (nitro-L-Arginine methyl ester) or the cyclooxygenase inhibitor (indomethacin) for 20 min, and the effects of norepinephrine and acetylcholine were re-evaluated. Results: Norepinephrine-induced vasoconstriction was enhanced after DHT treatment, but this effect was attenuated by calcitriol administration. Vasorelaxation of DHT-treated thoracic aortic rings was impaired, but this could be partly reversed by calcitriol application. Impaired NO-dependent vasorelaxation in DHT-treated animals was mostly reversed by concomitant calcitriol administration, but this effect was diminished by prostanoid-dependent vasoconstriction. Conclusions: These studies show that the enhanced sensitivity to vasoconstrictors and impaired NO-dependent vasorelaxation in hyperandrogenic PCOS rats could be partially reversed by calcitriol treatment.

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