Effects of vitamin D deficiency on proliferation and autophagy of ovarian and liver tissues in a rat model of polycystic ovary syndrome

Krisztina Lajtai, Csilla Terézia Nagy, Róbert Tarszabó, Rita Benkő, Leila Hadjadj, Réka Eszter Sziva, Dóra Gerszi, Bálint Bányai, Péter Ferdinandy, György László Nádasy, Zoltán Giricz, Eszter Mária Horváth, Szabolcs Várbíró

Research output: Contribution to journalArticle

Abstract

Aim: We aimed to examine the alterations of the insulin signaling pathway, autophagy, nitrative stress and the effect of vitamin D supplementation in the liver and ovaries of vitamin D deficient hyperandrogenic rats. Methods: FemaleWistar rats received eight weeks of transdermal testosterone treatment and lived on a low vitamin D diet (D-T+). Vitamin D supplementation was achieved by oral administration of vitamin D3 (D+T+). Sham-treated (D+T-) and vitamin D deficient animals (D-T-) served as controls. (N = 10-12 per group). Results: D-T+ animals showed decreased LC3 II levels in the liver and increased p-Akt/Akt and p-eNOS/eNOS ratios with decreased insulin receptor staining in the ovaries. Vitamin D supplementation prevented the increase of Akt phosphorylation in the ovaries. Vitamin D deficiency itself also led to decreased LC3 II levels in the liver and decreased insulin receptor staining in the ovaries. D-T+ group showed no increase in nitrotyrosine staining; however, the ovaries of D-T- rats and the liver of D+T+ animals showed increased staining intensity. Conclusion: Vitamin D deficiency itself might lead to disrupted ovarian maturation and autophagy malfunction in the liver. Preventing Akt phosphorylation may contribute to the beneficial effect of vitamin D treatment on ovarian function in hyperandrogenism.

Original languageEnglish
Article number471
JournalBiomolecules
Volume9
Issue number9
DOIs
Publication statusPublished - Sep 2019

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Keywords

  • Autophagy
  • Hyperandrogenism
  • Insulin resistance
  • Oxidative stress
  • Polycystic ovary syndrome (PCOS)
  • Vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Lajtai, K., Nagy, C. T., Tarszabó, R., Benkő, R., Hadjadj, L., Sziva, R. E., Gerszi, D., Bányai, B., Ferdinandy, P., Nádasy, G. L., Giricz, Z., Horváth, E. M., & Várbíró, S. (2019). Effects of vitamin D deficiency on proliferation and autophagy of ovarian and liver tissues in a rat model of polycystic ovary syndrome. Biomolecules, 9(9), [471]. https://doi.org/10.3390/biom9090471