Effects of tumor necrosis factor α (TNFα) on the phospholipid metabolism of Tetrahymena pyriformis

P. Kovács, L. Köhidai, G. Csaba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effect of (0.05 ng ml-1 and 0.1 ng ml-1) TNFα on the phospholipid metabolism of Tetrahymena pyriformis was studied. The amount of phosphatidyl choline (PC), phosphatidyl inositol (PI), phosphatidic acid (PA), phosphatidyl ethanolamine (PE), diacylglycerol (DAG), arachidonic acid (AA) and ceramide was higher, but the phosphatidyl inositol 4 phosphate (PIP) and phosphatidyl inositol bis-phosphate (PIP2) as well, as sphingomyelin (SM) content was lower in TNFα-treated cells than in the controls. In the culture medium (secreted forms) this situation was reversed. There were differences in the results gained by incorporation of [3H]-palmitic acid or 32P into the phospholipids. To control the functional effects of TNFα in Tetrahymena, the rate of cell division, the condensation of chromatin, the viability of cells and morphometrical values have been studied. The cytokine reduced cell growth, altered morphometric indices and increased chromatin condensation, however cell viability was not influenced. The results demonstrate the effects of TNFα at a low level of evolution, what is realized by changes in the phospolipid metabolism participating in signalling pathways.

Original languageEnglish
Pages (from-to)87-97
Number of pages11
JournalCell biochemistry and function
Volume16
Issue number2
DOIs
Publication statusPublished - Jun 1998

Keywords

  • Ceramide
  • Chromatin condensation
  • Phospholipids
  • Sphingomyelin
  • TNFα
  • Tetrahymena

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Effects of tumor necrosis factor α (TNFα) on the phospholipid metabolism of Tetrahymena pyriformis'. Together they form a unique fingerprint.

  • Cite this