Effects of TSH and anti-TSH receptor antibodies on the plasma membrane potential of polymorphonuclear granulocytes

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Abstract

Effects of thyrotropin hormone (TSH) and anti-TSH receptor antibodies on the plasma membrane potential of polymorphonuclear granulocytes (PMN) were analyzed by means of flow cytometry. Both TSH and the autoantibody caused a rapid, dose-dependent hyperpolarization of the plasma membrane of PMNs. TSH was also able to mask (revert) the depolarizing effect of a chemotactic peptide, fMLP, on PMNs. No detectable rise in the cytosolic free calcium level accompanied the observed hyperpolarization. Quinine, a blocker of Ca2+-activated and voltage-gated K+ channels did not affect the hyperpolarization by TSH and antibodies. Decreasing the [K+] gradient across the plasma membrane by valinomycin, however, blocked the hyperpolarizing effect. Peptide362-376 (derived from the extracellular domain of TSH receptor) also blocked the hyperpolarization induced by both TSH and anti-TSHR antibodies. These data suggest that the observed hyperpolarization is a specific, receptor-mediated early signal during interaction of PMNs with TSH or anti-TSHR antibodies.

Original languageEnglish
Pages (from-to)173-177
Number of pages5
JournalImmunology letters
Volume55
Issue number3
DOIs
Publication statusPublished - Mar 1 1997

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Keywords

  • K-permeability
  • autoantibodies
  • extrathyroid TSH receptors
  • granulocyt e activation
  • hyperpolarization

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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