Effects of TRPV1 receptor antagonists on stimulated iCGRP release from isolated skin of rats and TRPV1 mutant mice

G. Pethő, Iwona Izydorczyk, Peter W. Reeh

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Capsaicin antagonists including ruthenium red, capsazepine and iodo-resiniferatoxin (I-RTX) have recently been shown to inhibit the activation by noxious heat of the capsaicin receptor (TRPV1) expressed in non-neuronal host cells, and natively, in cultured dorsal root ganglion cells. Noxious heat has been shown to release immunoreactive calcitonin gene-related peptide (iCGRP) from the isolated rat skin. In this model, ruthenium red, I-RTX as well as capsazepine 10 μM caused no alteration in iCGRP release at 32°C by themselves whereas capsazepine 100 μM doubled it reversibly. In wild-type mice 100 μM capsazepine also stimulated iCGRP release while it was without effect in TRPV1 knockout littermates. In the rat skin, both ruthenium red and capsazepine (10/100 μM) reduced and abolished, respectively, capsaicin-induced iCGRP release while I-RTX (1/10 μM) was ineffective. Only ruthenium red 100 μM showed an unspecific effect inhibiting iCGRP release induced by KCl. Ruthenium red and capsazepine (10/100 μM) caused no significant alteration of iCGRP release induced by heat stimulation at 47°C. Employing 45°C stimulation intensity, capsazepine and I-RTX (in the higher concentrations) showed a significant facilitatory effect on the heat response suggesting a partial agonistic action of the compounds. It is concluded that noxious heat-induced iCGRP release in the isolated rat skin occurs through a mechanism that is not inhibited by TRPV1 antagonism reflecting a different pharmacological profile of noxious heat transduction in terminals of sensory neurons compared to that in cultured cell bodies and TRPV1-transfected host cells.

Original languageEnglish
Pages (from-to)284-290
Number of pages7
JournalPain
Volume109
Issue number3
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Calcitonin Gene-Related Peptide
Ruthenium Red
Hot Temperature
Skin
Capsaicin
TRPV Cation Channels
Spinal Ganglia
Sensory Receptor Cells
capsazepine
TRPV1 receptor
Cultured Cells
Pharmacology
iodoresiniferatoxin

Keywords

  • Capsazepine
  • Iodo-resiniferatoxin
  • Nociception
  • Noxious heat
  • Ruthenium red
  • Transgenic
  • TRPV1

ASJC Scopus subject areas

  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Neuroscience(all)
  • Pharmacology
  • Clinical Psychology

Cite this

Effects of TRPV1 receptor antagonists on stimulated iCGRP release from isolated skin of rats and TRPV1 mutant mice. / Pethő, G.; Izydorczyk, Iwona; Reeh, Peter W.

In: Pain, Vol. 109, No. 3, 06.2004, p. 284-290.

Research output: Contribution to journalArticle

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