Effects of Trolox C and SIN-1 on arachidonic acid metabolism and on cyclic GMP formation in leukocytes

Asko Riutta, Juha Alanko, I. Muchá, Heikki Vapaatalo

Research output: Contribution to journalArticle

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Abstract

The effects of Trolox C (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a vitamin E analogue, (60-900 μM) and SIN-1 (3-morpholino syndonimine), a nitric oxide donor, (30-3000 μM) on arachidonic acid metabolism and on cyclic GMP formation in calcium ionophore A23187 (calcimycin)-stimulated human polymorphonuclear leukocytes were investigated. Trolox C elicited a dose dependent decrease in leukotriene B4 levels and increase in prostaglandin E2 levels but did not affect cyclic GMP levels. SIN-1 dose dependently inhibited leukotriene B4 and stimulated prostaglandin E2 and cyclic GMP formation. Dibutyryl cyclic GMP did not affect the formation of leukotriene B4 and prostaglandin E2. Trolox C (180 μM), which itself had no effect on cyclic GMP levels, enhanced the effect of SIN-1 (100 μM) on cyclic GMP levels more than 5-fold. The effects of SIN-1 on arachidonic acid metabolism seem to be independent of cyclic GMP and are probably due to nitric oxide. In this experimental model both Trolox C and SIN-1 have similar actions on the prostaglandin/leukotriene ratio, and Trolox C potentiates the SIN-1-induced increase in cyclic GMP levels.

Original languageEnglish
Pages (from-to)217-222
Number of pages6
JournalEuropean Journal of Pharmacology
Volume262
Issue number3
DOIs
Publication statusPublished - Sep 12 1994

Fingerprint

Cyclic GMP
Arachidonic Acid
Leukocytes
Leukotriene B4
Dinoprostone
Calcimycin
Dibutyryl Cyclic GMP
Morpholinos
Leukotriene C4
Nitric Oxide Donors
Calcium Ionophores
6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid
Vitamin E
Prostaglandins
Nitric Oxide
Neutrophils
Theoretical Models

Keywords

  • Antioxidant
  • cGMP
  • Leukocyte
  • Leukotriene
  • Nitric oxide (NO)
  • Prostaglandin
  • SIN (3-morpholino sydnonimine)
  • Trolox

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Effects of Trolox C and SIN-1 on arachidonic acid metabolism and on cyclic GMP formation in leukocytes. / Riutta, Asko; Alanko, Juha; Muchá, I.; Vapaatalo, Heikki.

In: European Journal of Pharmacology, Vol. 262, No. 3, 12.09.1994, p. 217-222.

Research output: Contribution to journalArticle

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