Effects of trifluoperazine on the cholinergic function of the hippocampus of the rat

Research output: Contribution to journalArticle

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Abstract

The changes induced in the level and turnover rate (TRACh) of acetylcholine (ACh) in the hippocampus and in the in vitro release of [3H]ACh by intracerebroventricular injection of trifluoperazine (TFP) were studied. Rats were killed by microwave irradiation at various times after treatment with drug or vehicle and the levels of ACh were measured by gas chromatography. After the administration of 100 μg trifluoperazine, the content of ACh of the whole hippocampus increased from 24.0 ± 2.9 to 35.2 ± 4.2 nmols/g wet weight over a 60 min period, while the turnover rate was markedly decreased (from 0.86 to 0.53 nmols/min/g wet tissue weight). The potassium-evoked release of [3H]ACh from slices of hippocampus under in vitro conditions was decreased in a concentration-dependent manner by pre-incubation with 50 and 100 μM of trifluoperazine for 60 min. The results support the assumption that trifluoperazine can alter neurotransmission, possibly by inhibiting the calcium-binding protein calmodulin, and/or by interfering with cell metbolism.

Original languageEnglish
Pages (from-to)439-443
Number of pages5
JournalNeuropharmacology
Volume26
Issue number5
DOIs
Publication statusPublished - 1987

Fingerprint

Trifluoperazine
Cholinergic Agents
Acetylcholine
Hippocampus
Weights and Measures
Calcium-Binding Proteins
Calmodulin
Microwaves
Synaptic Transmission
Gas Chromatography
Potassium
Injections
Pharmaceutical Preparations

Keywords

  • ACh level, turnover rate, release
  • trifluoperazine, hippocampus

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

Effects of trifluoperazine on the cholinergic function of the hippocampus of the rat. / Budai, D.; Kása, P.

In: Neuropharmacology, Vol. 26, No. 5, 1987, p. 439-443.

Research output: Contribution to journalArticle

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