Effects of the xanthine oxidase system on cardiac function in anaesthetised rats

Sarra K. Laycock, John McMurray, Kathleen A. Kane, J. Parratt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This investigation aimed to determine whether contractile dysfunction of the myocardium could be produced upon generation of free radicals in the anaesthetised rat. The enzyme xanthine oxidase, combined with its substrate purine and an iron source, was used to generate free radicals in the venous circulation. The suspended form of xanthine oxidase, with substrate, produced a transient, significant depression in the contractile indices dP dt-1 max and dP dt-1 P-1 and arterial blood pressure, 1146 ± 87 mm Hg s-1, 9 ± 1 s-1, and 18 ± 1 mm Hg, respectively. This could not be attenuated by the enzymatic free radical scavengers superoxide dismutase and catalase. Furthermore, the suspended xanthine oxidase alone or its vehicle were able to produce a similar effect to that of the complete free-radical-generating system. The maximum soluble dose of the crystalline form of the enzyme when employed in the generating system had no effect upon administration despite its production of superoxide radicals in vitro. These results suggest that the haemodynamic effects of the free-radical-generating system containing the suspended form of xanthine oxidase were due to the effects of its vehicle and that the free-radical-generating system containing the crystalline form of the enzyme did not produce sufficient free radicals in vivo to modify myocardial contractility.

Original languageEnglish
Pages (from-to)249-255
Number of pages7
JournalFree Radical Biology and Medicine
Volume15
Issue number3
DOIs
Publication statusPublished - 1993

Fingerprint

Xanthine Oxidase
Free Radicals
Rats
Enzymes
Crystalline materials
Free Radical Scavengers
Blood pressure
Hemodynamics
Substrates
Superoxides
Catalase
Superoxide Dismutase
Myocardium
Arterial Pressure
Iron

Keywords

  • Anaesthetised rats
  • Free radicals
  • Myocardial function
  • Purine
  • Xanthine oxidase

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)
  • Toxicology

Cite this

Effects of the xanthine oxidase system on cardiac function in anaesthetised rats. / Laycock, Sarra K.; McMurray, John; Kane, Kathleen A.; Parratt, J.

In: Free Radical Biology and Medicine, Vol. 15, No. 3, 1993, p. 249-255.

Research output: Contribution to journalArticle

Laycock, Sarra K. ; McMurray, John ; Kane, Kathleen A. ; Parratt, J. / Effects of the xanthine oxidase system on cardiac function in anaesthetised rats. In: Free Radical Biology and Medicine. 1993 ; Vol. 15, No. 3. pp. 249-255.
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