Effects of the venom of the brown bullhead catfish (Ameiurus nebulosus) on isolated smooth muscles

L. Barthó, Zsolt Sándor, Timea Bencsik

Research output: Contribution to journalArticle

Abstract

Aqueous extract of the spines of the brown bullhead catfish (Ameiurus nebulosus Lesueur, 1819) caused contraction of the Guinea-pig small intestine in vitro, a widely-used preparation in pharmacology. The action is dependent on extracellular Ca2+, and probably takes place on the smooth muscle cells. Mouse gastrointestinal preparations were also contracted by the extract. Stings by the spines of this fish species causes a painful sensation in man. We tested the effect of an extract of spines in isolated organ experiments on innervated smooth muscle preparations. In the Guinea-pig ileum, the response to the extract was abolished by the Ca2+-channel blocker nifedipine, but only slightly reduced by atropine (a muscarine receptor antagonist) or tetrodotoxin (TTX; a blocker axonal conduction) or antagonists for P2X purinocep-tors. Blocking of serotonin or histamine H1 receptors, tachykinin NK1 receptors, functional impairment of capsaicin-sensitive sensory nerve endings or inhibition of cyclo-oxygenases failed to influence the contractile effect of the extract. No inhibitory action of the extract was detected on the ileum subject to electrical motor nerve stimulation.

Original languageEnglish
Pages (from-to)135-143
Number of pages9
JournalActa Biologica Hungarica
Volume69
Issue number2
DOIs
Publication statusPublished - Jun 1 2018

Fingerprint

Ictaluridae
venom
Venoms
pig
Smooth Muscle
Muscle
muscle
Spine
Muscarine
Ileum
Tachykinins
Histamine H1 Receptors
Capsaicin
Tetrodotoxin
Guinea Pigs
Nifedipine
Prostaglandin-Endoperoxide Synthases
Atropine
Tachykinin Receptors
Fish

Keywords

  • Brown bullhead catfish
  • Smooth muscle effects
  • Venom

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Effects of the venom of the brown bullhead catfish (Ameiurus nebulosus) on isolated smooth muscles. / Barthó, L.; Sándor, Zsolt; Bencsik, Timea.

In: Acta Biologica Hungarica, Vol. 69, No. 2, 01.06.2018, p. 135-143.

Research output: Contribution to journalArticle

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