Effects of the neuropeptides substance P, calcitonin gene-related peptide and α-melanocyte-stimulating hormone on the IL-8/IL-8 receptor system in a cultured human keratinocyte cell line and dermal fibroblasts

Mária Kiss, Lajos Kemény, Rolland Gyulai, Günter Michel, Sándor Husz, Réka Kovács, Attila Dobozy, Thomas Ruzicka

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The neuropeptides substance P (SP), calcitonin gene-related peptide (CGRP) and α-melanocyte-stimulating hormone (α-MSH) are known to be able to regulate the production of cytokines in the skin. Since IL-8 plays an important role in cutaneous inflammation, the effects of SP, CGRP and α-MSH on the IL-8/IL-8 receptor (IL-8RA) systems of these cell types were studied. Cultures of human dermal fibroblasts and an immortalized keratinocyte cell line HaCaT were treated with 10-8 M SP, CGRP or α-MSH. The results demonstrated that these neuropeptides have different effects on the IL-8 and IL-8RA expressions of the cells. SP and CGRP upregulated the IL-8RA mRNA expression in HaCaT cells, but had no influence on their IL-8 production, whereas, α-MSH had no effect on either the IL-8 or the IL-8RA mRNA expression in HaCaT cells. In contrast, α-MSH resulted in a time-dependent induction of the IL-8 mRNA expression in dermal fibroblasts. This induction was already detectable after 6 h, and after 12 h there was a 5-fold change in comparison with the controls. The IL-8 content of the supernatant was also increased, with a maximum at 48 h after α-MSH treatment. The data established in the present study support the notion that neuropeptides can directly modulate the IL-8/IL-8RA system of keratinocytes and fibroblasts.

Original languageEnglish
Pages (from-to)557-567
Number of pages11
JournalInflammation
Volume23
Issue number6
DOIs
Publication statusPublished - Nov 17 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Effects of the neuropeptides substance P, calcitonin gene-related peptide and α-melanocyte-stimulating hormone on the IL-8/IL-8 receptor system in a cultured human keratinocyte cell line and dermal fibroblasts'. Together they form a unique fingerprint.

  • Cite this