Effects of the mammalian vasoconstrictor peptide, endothelin-1, on Tetrahymena pyriformis GL, and the immunocytological detection of endogenous endothelin-like activity

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Abstract

The vasoconstrictor endothelin-1 (ET-1) is shown to have significant physiological effects on a unicellular organism, Tetrahymena pyriformis. These responses include: (1) A significant increase in intracellular [Ca2+] induced by 10-10 M ET-1; (2) Increased chemotaxis, maximal at 10-10 M; and (3) A small inhibition of proliferation at the 10-13-10-12 M concentration range. Immunocytochemical detection of endogenous ET-1 using rabbit antibodies directed against human or porcine ET-1 indicates that this is a further example of the widening group of vertebrate hormones now known to be synthesized by Tetrahymena. These observations suggest that hormones are of considerable antiquity in their phylogenetic appearance and have been highly conserved throughout evolution.

Original languageEnglish
Pages (from-to)311-316
Number of pages6
JournalComparative Biochemistry and Physiology. Part C, Comparative
Volume111
Issue number2
DOIs
Publication statusPublished - 1995

Fingerprint

Tetrahymena pyriformis
Endothelins
Vasoconstrictor Agents
Endothelin-1
Peptides
Endothelin-2
Hormones
Tetrahymena
Chemotaxis
Vertebrates
Swine
Rabbits
Antibodies

Keywords

  • Chemotaxis
  • Endothelin-1
  • Growth
  • Immunocytology
  • Microwave fixation
  • Tetrahymena, Evolution

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

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title = "Effects of the mammalian vasoconstrictor peptide, endothelin-1, on Tetrahymena pyriformis GL, and the immunocytological detection of endogenous endothelin-like activity",
abstract = "The vasoconstrictor endothelin-1 (ET-1) is shown to have significant physiological effects on a unicellular organism, Tetrahymena pyriformis. These responses include: (1) A significant increase in intracellular [Ca2+] induced by 10-10 M ET-1; (2) Increased chemotaxis, maximal at 10-10 M; and (3) A small inhibition of proliferation at the 10-13-10-12 M concentration range. Immunocytochemical detection of endogenous ET-1 using rabbit antibodies directed against human or porcine ET-1 indicates that this is a further example of the widening group of vertebrate hormones now known to be synthesized by Tetrahymena. These observations suggest that hormones are of considerable antiquity in their phylogenetic appearance and have been highly conserved throughout evolution.",
keywords = "Chemotaxis, Endothelin-1, Growth, Immunocytology, Microwave fixation, Tetrahymena, Evolution",
author = "L. Kőhidai and G. Csaba",
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AU - Kőhidai, L.

AU - Csaba, G.

PY - 1995

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N2 - The vasoconstrictor endothelin-1 (ET-1) is shown to have significant physiological effects on a unicellular organism, Tetrahymena pyriformis. These responses include: (1) A significant increase in intracellular [Ca2+] induced by 10-10 M ET-1; (2) Increased chemotaxis, maximal at 10-10 M; and (3) A small inhibition of proliferation at the 10-13-10-12 M concentration range. Immunocytochemical detection of endogenous ET-1 using rabbit antibodies directed against human or porcine ET-1 indicates that this is a further example of the widening group of vertebrate hormones now known to be synthesized by Tetrahymena. These observations suggest that hormones are of considerable antiquity in their phylogenetic appearance and have been highly conserved throughout evolution.

AB - The vasoconstrictor endothelin-1 (ET-1) is shown to have significant physiological effects on a unicellular organism, Tetrahymena pyriformis. These responses include: (1) A significant increase in intracellular [Ca2+] induced by 10-10 M ET-1; (2) Increased chemotaxis, maximal at 10-10 M; and (3) A small inhibition of proliferation at the 10-13-10-12 M concentration range. Immunocytochemical detection of endogenous ET-1 using rabbit antibodies directed against human or porcine ET-1 indicates that this is a further example of the widening group of vertebrate hormones now known to be synthesized by Tetrahymena. These observations suggest that hormones are of considerable antiquity in their phylogenetic appearance and have been highly conserved throughout evolution.

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KW - Microwave fixation

KW - Tetrahymena, Evolution

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