Effects of the cholinotoxin, AF 64A, on neuronal trace-metal distribution in the rat hippocampus and neocortex

P. Szerdahelyi, P. Kása, A. Fisher, I. Hanin

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Abstract

Ethylcholine mustard aziridinium ion (AF64A) is a neurotoxin which is specific for cholinergic nerve terminals. Besides its effects on elements of the acetylcholine system, we observed that, after 2 and 8 days, a single 20-nmol intracerebroventricular dose altered the Timm's staining of certain regions of the central nervous system and reduced the tissue levels of trace metals. In the hippocampal formation, there was a considerable decrease in the staining of the neuropil of the stratum radiatum and stratum oriens, which contain cholinergic nerve terminals. A reduction in staining was also demonstrated in the perikarya of cortical pyramidal cells. The diminished trace-metal level in both regions was confirmed by quantitative measurements of zinc and copper levels. A similar reduction was not observed at a lower dose (8 nmol) of the cholinotoxin. The results led to the conclusion that AF64A may cause the decrease of the trace-metal content of the postsynaptic neurons through an indirect mechanism.

Original languageEnglish
Pages (from-to)497-500
Number of pages4
JournalHistochemistry
Volume81
Issue number5
DOIs
Publication statusPublished - Sep 1984

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Neocortex
Hippocampus
Metals
Staining and Labeling
Cholinergic Agents
Neuropil
Pyramidal Cells
Neurotoxins
Acetylcholine
Zinc
Copper
Central Nervous System
Ions
Neurons
ethylcholine aziridinium

ASJC Scopus subject areas

  • Anatomy

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Effects of the cholinotoxin, AF 64A, on neuronal trace-metal distribution in the rat hippocampus and neocortex. / Szerdahelyi, P.; Kása, P.; Fisher, A.; Hanin, I.

In: Histochemistry, Vol. 81, No. 5, 09.1984, p. 497-500.

Research output: Contribution to journalArticle

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